Genomics has provided us with vast amounts of data and thus, the challenge to identify and characterize gene products. Proteomics analysis, using methods such as yeast two-hybrid screenings, isoelectric focusing, and mass spectroscopy, generate potentially useful information. To determine functional relationships between and among proteins, however, the initial data for putative protein interactions must first be validated. One technique, which is considered the gold standard, is coimmunoprecipitation.
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References
Duzhyy, D., Harvey, M., Sokolowski, B. (2005) A secretory-type protein, containing a pentraxin domain, interacts with an A-type K+channel. J. Biol. Chem. 280, 15165–15172.
Markham, K., Bai, Y., Schmitt-Ulms, G. (2007) Co-immunoprecipitations revisited: an update on experimental concept and their implementation for sensitive interactome investigations of endogenous proteins. Anal. Bioanal. Chem. 389, 461–473.
Bhairi, S. M. (2001) A Guide to the Properties and Uses of Detergents in Biology and Biochemistry. Calbiochem-Novabiochem Corp., La Jolla, CA, 41pp.
Carraway, K. L., Carraway, C. A. C. (Eds.) (1999) Cytoskeleton Signalling and Cell Regulation. Oxford University Press, New York, NY, 287pp.
Acknowledgments
This work was supported by NIDCD grant DC04295 to B. H. A. S.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Harvey, M.C., Sokolowski, B.H. (2009). In Vivo Verification of Protein Interactions in the Inner Ear by Coimmunoprecipitation. In: Sokolowski, B. (eds) Auditory and Vestibular Research. Methods in Molecular Biology™, vol 493. Humana Press. https://doi.org/10.1007/978-1-59745-523-7_18
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DOI: https://doi.org/10.1007/978-1-59745-523-7_18
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