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Auditory and Vestibular Research

Methods and Protocols

  • Bernd Sokolowski

Part of the Methods in Molecular Biology™ book series (MIMB, volume 493)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Nucleic Acid Protocols

    1. Front Matter
      Pages 1-1
    2. Casilda Trujillo-Provencio, TuShun R. Powers, David R. Sultemeier, Elba E. Serrano
      Pages 3-20
    3. Ana E. Vázquez, Liping Nie, Ebenezer N. Yamoah
      Pages 21-29
    4. John V. Brigande, Samuel P. Gubbels, David W. Woessner, Jonathan J. Jungwirth, Catherine S. Bresee
      Pages 125-139
    5. Kazuo Oshima, Pascal Senn, Stefan Heller
      Pages 141-162
    6. Long-Sheng Chang, D. Bradley Welling
      Pages 163-177
    7. Laura Nistico, Armin Gieseke, Paul Stoodley, Luanne Hall-Stoodley, Joseph E. Kerschner, Garth D. Ehrlich
      Pages 191-213
    8. Hannie Kremer, Frans P.M. Cremers
      Pages 215-239
  3. Amino Acid Protocols

    1. Front Matter
      Pages 239-239
    2. Jung-Bum Shin, James Pagana, Peter G. Gillespie
      Pages 241-255
    3. Thandavarayan Kathiresan, Margaret C. Harvey, Bernd H. A. Sokolowski
      Pages 269-286
    4. Margaret C. Harvey, Bernd H.A. Sokolowski
      Pages 299-310
    5. Dennis G. Drescher, Marian J. Drescher, Neeliyath A. Ramakrishnan
      Pages 323-343
  4. Imaging Protocols

    1. Front Matter
      Pages 367-367
    2. Heather Jensen-Smith, Benjamin Currall, Danielle Rossino, LeAnn Tiede, Michael Nichols, Richard Hallworth
      Pages 369-379
    3. Gregory I. Frolenkov
      Pages 381-399
    4. Michio Murakoshi, Hiroshi Wada
      Pages 401-413
  5. Back Matter
    Pages 415-421

About this book

Introduction

While early studies of the auditory/vestibular sciences provided insights into the anatomy and neurophysiology of these systems and produced a prosthetic cochlear implant, the rise of molecular biology now permits a clear examination of the genetic basis for various hearing and balance disorders. In Auditory and Vestibular Research: Methods and Protocols, specialists in the field describe current RNA, protein, and imaging protocols that have provided insights into genetic regulation as well as a greater understanding of the genes and pathogens involved in diseases of the ear. This overview utilizes both mammalian and non-mammalian animal models as well as techniques applicable to clinical studies in order to best provide an up-to-date perspective on basic research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Auditory and Vestibular Research: Methods and Protocols is an ideal guide to the field and a useful tool for exploring genes and proteins in other systems as well, especially systems in which tissues are scarce and a comparative approach lends itself to discovering the underlying causes of human disorders.

Keywords

cells gene expression gene transfer genes hybridization microscopy molecular biology physiology regulation tissue

Editors and affiliations

  • Bernd Sokolowski
    • 1
  1. 1.University of South Florida, College of MedicineTampaUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-523-7
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-62-6
  • Online ISBN 978-1-59745-523-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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