Auditory and Vestibular Research

1. Front Matter

   Pages i-xii

   PDF

2. Nucleic Acid Protocols

   1. Front Matter

      Pages 1-1

      PDF

   2. RNA Isolation from Xenopus Inner Ear Sensory Endorgans for Transcriptional Profiling and Molecular Cloning

      • Casilda Trujillo-Provencio, TuShun R. Powers, David R. Sultemeier, Elba E. Serrano

      Pages 3-20

   3. Synthesis of Biotin-Labeled RNA for Gene Expression Measurements Using Oligonucleotide Arrays

      • Ana E. Vázquez, Liping Nie, Ebenezer N. Yamoah

      Pages 21-29

   4. In situ Hybridization Approach to Study mRNA Expression and Distribution in Cochlear Frozen Sections

      • Hakim Hiel

      Pages 31-46

   5. Lineage Analysis of Inner Ear Cells Using Genomic Tags for Clonal Identification

      • Takunori Satoh, Donna M. Fekete

      Pages 47-64

   6. Genetic Fate-Mapping Approaches: New Means to Explore the Embryonic Origins of the Cochlear Nucleus

      • Jun Chul Kim, Susan M. Dymecki

      Pages 65-85

   7. The Practical Use of Cre and loxP Technologies in Mouse Auditory Research

      • Yiling Yu, Jian Zuo

      Pages 87-102

   8. Helios® Gene Gun–Mediated Transfection of the Inner Ear Sensory Epithelium

      • Inna A. Belyantseva

      Pages 103-124

   9. Electroporation-Mediated Gene Transfer to the Developing Mouse Inner Ear

      • John V. Brigande, Samuel P. Gubbels, David W. Woessner, Jonathan J. Jungwirth, Catherine S. Bresee

      Pages 125-139

   10. Isolation of Sphere-Forming Stem Cells from the Mouse Inner Ear

       • Kazuo Oshima, Pascal Senn, Stefan Heller

       Pages 141-162

   11. Molecular Biology of Vestibular Schwannomas

       • Long-Sheng Chang, D. Bradley Welling

       Pages 163-177

   12. Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis Media Causing Pathogens

       • Jonathan C. Thomas, Melinda M. Pettigrew

       Pages 179-190

   13. Fluorescence “In Situ” Hybridization for the Detection of Biofilm in the Middle Ear and Upper Respiratory Tract Mucosa

       • Laura Nistico, Armin Gieseke, Paul Stoodley, Luanne Hall-Stoodley, Joseph E. Kerschner, Garth D. Ehrlich

       Pages 191-213

   14. Positional Cloning of Deafness Genes

       • Hannie Kremer, Frans P.M. Cremers

       Pages 215-239

3. Amino Acid Protocols

   1. Front Matter

      Pages 239-239

      PDF

   2. Twist-Off Purification of Hair Bundles

      • Jung-Bum Shin, James Pagana, Peter G. Gillespie

      Pages 241-255

   3. Yeast Two-Hybrid Screening to Test for Protein–Protein Interactions in the Auditory System

      • Dhasakumar S. Navaratnam

      Pages 257-268

   4. The Use of 2-D Gels to Identify Novel Protein–Protein Interactions in the Cochlea

      • Thandavarayan Kathiresan, Margaret C. Harvey, Bernd H. A. Sokolowski

      Pages 269-286

   5. Identification of Functionally Important Residues/Domains in Membrane Proteins Using an Evolutionary Approach Coupled with Systematic Mutational Analysis

      • Lavanya Rajagopalan, Fred A. Pereira, Olivier Lichtarge, William E. Brownell

      Pages 287-297

Hearing is a sensory modality critical to both language and cognitive development. In its absence, and without sensory input through another modality, such as the manual/visual modality of sign language, cognitive and language development can be severely impaired in the earliest formative years of a child. In its endeavor to d- cover the mechanisms underlying audition, the ?eld of auditory science has provided rich comparative physiological studies, allowing insights into both the micromecha- cal and electrochemical world of this system. For many years, the auditory/vestibular sciences have been in?uenced by the discoveries of electrical engineers and sensory physiologists, who have provided insights into the functions of this dynamic system. The early discoveries in these ?elds, as well as advancements in microprocessing and materials technologies, provided a means whereby hearing could be regained partly through the use of a bionic device, known as a cochlear implant. Presently, this device and the auditory brainstem implant are the only ones to prosthetically replace brain function. With the advent of molecular biology tools, such as RT-PCR, the auditory and vestibular ?elds have made great strides in understanding the genetic basis for various hearing and balance disorders over the past ?fteen to twenty years. These technologies permitted the discovery of genes that control inner ear structure and function by ov- coming the hurdle of working with small amounts of tissue, as found in the inner ear.

• cells
• gene expression
• gene transfer
• genes
• hybridization
• microscopy
• molecular biology
• physiology
• regulation
• tissue

• University of South Florida, College of Medicine, Tampa, USA

  Bernd Sokolowski

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