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Variable Number Tandem Repeat Typing of Bacteria

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Comparative Genomics

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 396))

Summary

Analysis of bacterial genomes revealed a high percentage of DNA consisting of repeats, in which DNA motifs existed in multiple copies. Study of these DNA motifs has resulted in the development of variable number tandem repeat (VNTR) or multilocus variant-repeat analysis (MLVA) assays, which have shown to be valuable bacterial typing methods, especially in relation to disease outbreaks. The VNTR-based assay is based on direct PCR amplification of a specific locus, which is well defined. The range and polymorphism index of each locus can be calculated. This chapter describes the VNTR analysis of Neisseria meningitides-based on separation in low resolution media agarose, and VNTR analysis of Salmonella enterica subsp. enterica serovars Typhimurium-based on high resolution capillary electrophoresis.

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Authors and Affiliations

  1. Norwegian Institute of Public Health, Norway

    Siamak P. Yazdankhah & Bjørn-Arne Lindstedt

Authors
  1. Siamak P. Yazdankhah
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  2. Bjørn-Arne Lindstedt
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Editor information

Editors and Affiliations

  1. Bioinformatics Program and Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI

    Nicholas H. Bergman

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© 2007 Humana Press Inc.

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Yazdankhah, S.P., Lindstedt, BA. (2007). Variable Number Tandem Repeat Typing of Bacteria. In: Bergman, N.H. (eds) Comparative Genomics. Methods In Molecular Biology™, vol 396. Humana Press. https://doi.org/10.1007/978-1-59745-515-2_25

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  • DOI: https://doi.org/10.1007/978-1-59745-515-2_25

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-934115-37-4

  • Online ISBN: 978-1-59745-515-2

  • eBook Packages: Springer Protocols

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