Summary
This method allows high-throughput qualitative screening to identify seeds containing a transgene product in endosperm tissue. It is particularly useful for determining genetic segregation ratios or identifying seeds to be advanced in a breeding program. Tissue printing is used to avoid time-consuming extraction steps. Antibody-based detection of the transgene product makes this method suitable to any transgene product for which a specific antibody is available. It is possible to screen thousands of seeds per week using this method.
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Acknowledgments
The author wishes to thank Mrs. Merinda Struthers for technical assistance.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Scott, M.P. (2009). Tissue-Print Immunodetection of Transgene Products in Endosperm for High-Throughput Screening of Seeds. In: Scott, M.P. (eds) Transgenic Maize. Methods in Molecular Biology™, vol 526. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-494-0_10
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DOI: https://doi.org/10.1007/978-1-59745-494-0_10
Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-59745-494-0
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