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Characterization of O-Linked Saccharides on Glycoproteins

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Pichia Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 389))

Abstract

Recombinant and native proteins of Pichia pastoris can be O-mannosylated on serine and threonine residues, allowing further elongation reactions to generate short O-linked oligosaccharides of mannose. Methods for release from the protein with alkaline β-elimination with or without reduction of the released saccharides, and for subsequent chromatographic and enzymatic characterization of these saccharides are described.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN

    Roger K. Bretthauer

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  1. Roger K. Bretthauer
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Editor information

Editors and Affiliations

  1. Keck Graduate Institute of Applied Life Sciences, Claremont, CA

    James M. Cregg

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© 2007 Humana Press Inc., Totowa, NJ

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Bretthauer, R.K. (2007). Characterization of O-Linked Saccharides on Glycoproteins. In: Cregg, J.M. (eds) Pichia Protocols. Methods in Molecular Biology, vol 389. Humana Press. https://doi.org/10.1007/978-1-59745-456-8_8

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  • DOI: https://doi.org/10.1007/978-1-59745-456-8_8

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-429-6

  • Online ISBN: 978-1-59745-456-8

  • eBook Packages: Springer Protocols

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