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Pichia Protocols

  • James M. Cregg

Part of the Methods in Molecular Biology book series (MIMB, volume 389)

Table of contents

  1. Front Matter
    Pages i-xi
  2. James M. Cregg
    Pages 1-10
  3. Joan Lin-Cereghino, Geoff P. Lin-Cereghino
    Pages 11-25
  4. James M. Cregg
    Pages 27-42
  5. Wenhui Zhang, Mehmet Inan, Michael M. Meagher
    Pages 43-63
  6. Mehmet Inan, Sarah A. Fanders, Wenhui Zhang, Peter J. Hotez, Bin Zhan, Michael M. Meagher
    Pages 65-75
  7. Jicai Huang, Rick Barent, Mehmet Inan, Mark Gouthro, Virginia P. Roxas, Leonard A. Smith et al.
    Pages 77-98
  8. Sandra E. Ríos, Erin M. Giaccone, Tillman U. Gerngross
    Pages 99-106
  9. Wouter Vervecken, Nico Callewaert, Vladimir Kaigorodov, Steven Geysens, Roland Contreras
    Pages 119-138
  10. Huijuan Li, Robert G. Miele, Teresa I. Mitchell, Tillman U. Gerngross
    Pages 139-149
  11. Eric Rodriguez
    Pages 151-164
  12. Anna M. Larsson, T. Alwyn Jones
    Pages 165-174
  13. Ilya Tolstorukov, James M. Cregg
    Pages 189-201
  14. Laura A. Schroder, Benjamin S. Glick, William A. Dunn Jr.
    Pages 203-218
  15. Sebastien Leon, Ivet Suriapranata, Mingda Yan, Naganand Rayapuram, Amar Patel, Suresh Subramani
    Pages 219-237
  16. Jean-Claude Farre, Kanae Shirahama-Noda, Lan Zhang, Keith Booher, Suresh Subramani
    Pages 239-249
  17. Back Matter
    Pages 261-268

About this book

Introduction

The use of Pichia Pastoris as a recombinant protein system has increased greatly in popularity over the years. In Pichia Protocols 2nd Edition, the author focuses on new information that has come to light in recent years but repeats key basic methods so that researchers are not absolutely dependent on having both editions in order to utilize the system. The chapters in this indispensable laboratory aid cover a wide range of topics associated with Pichia Pastoris. The use of fermentors to grow Pichia Pastoris and good advice on how to go about the initial processing of material from these cultures is discussed. An extensive discussion on how to formally analyze mutations in essential P. pastoris genes is also presented. The final chapters are for cell biologists who are for cell biologists using the Pichia Pastoris system to investigate protein interactions, or to localize proteins in yeast cells. Pichia Protocols 2nd Edition, is an essential tool for researchers working with the Pichia Pastoris system

Keywords

Expression Fermentation electron microscopy genes genetics microscopy

Editors and affiliations

  • James M. Cregg
    • 1
  1. 1.Keck Graduate Institute of Applied Life SciencesClaremont

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-456-8
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-429-6
  • Online ISBN 978-1-59745-456-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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