Summary
The progress made in DNA marker technology has been remarkable and exciting in recent years. DNA markers have proved valuable tools in various analyses in plant breeding, for example, early generation selection, enrichment of complex F1s, choice of donor parent in backcrossing, recovery of recurrent parent genotype in backcrossing, linkage block analysis and selection. Other main areas of applications of molecular markers in plant breeding include germplasm characterization/fingerprinting, determining seed purity, systematic sampling of germplasm, and phylogenetic analysis. Molecular markers, thus, have proved powerful tools in replacing the bioassays and there are now many examples available to show the efficacy of such markers. We have illustrated some basic concepts and methodology of applying molecular markers for enhancing the selection efficiency in plant breeding. Some successful examples of product developments of molecular breeding have also been presented.
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Acknowledgements
Authors (RKV, DAH, SNN) are grateful to Generation Challenge Programme (GCP) of Consultative Group on International Agriculture Research (CGIAR), Indo–US Agricultural Knowledge Initiative (AKI), and National Fund of Indian Council of Agricultural Research (ICAR) and Department of Biotechnology (DBT), Government of India for financial support of their research. SNN is thankful to councl of Scientific and Industrail Research (CSIR), Government of India for Sponsoring fellowship.
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Varshney, R., Hoisington, D., Nayak, S., Graner, A. (2009). Molecular Plant Breeding: Methodology and Achievements. In: Gustafson, J., Langridge, P., Somers, D. (eds) Plant Genomics. Methods in Molecular Biology™, vol 513. Humana Press. https://doi.org/10.1007/978-1-59745-427-8_15
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