Summary
In the post-genomic era, it is necessary to adapt methods for gene expression and functional analyses to more high-throughput levels of processing. mRNA in situ hybridization (ISH) remains a powerful tool for obtaining information regarding a gene’s temporal and spatial expression pattern and can therefore be used as a starting point to define the function of a gene or a whole set of genes. We have deconstructed ‘traditional’ ISH techniques described for a range of organisms and developed protocols for ISH that adapt and integrate a degree of automation to standardized and shortened protocols. We have adapted this technique as a high-throughput means of gene expression analysis on wax-embedded plant tissues and also on whole-mount tissues. We have used wax-embedded wheat grains and Arabidopsis floral meristems and whole-mount Arabidopsis roots as test systems and show that it is capable of highly parallel processing.
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Drea, S., Derbyshire, P., Koumproglou, R., Dolan, L., Doonan, J., Shaw, P. (2009). In situ Analysis of Gene Expression in Plants. In: Gustafson, J., Langridge, P., Somers, D. (eds) Plant Genomics. Methods in Molecular Biology™, vol 513. Humana Press. https://doi.org/10.1007/978-1-59745-427-8_12
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DOI: https://doi.org/10.1007/978-1-59745-427-8_12
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