Summary
Gene transfer into mature T cells with gammaretroviral vectors requires prestimulation, as only mitotic cells are susceptible to integration of the gammaretroviral proviral genome. Costimulation via the CD3/ TCR complex and a second costimulatory molecule, such as CD28 was found to better preserve functionality of the T lymphocytes during ex vivo expansion than stimulation with anti-CD3 alone. The protocols described here for prestimulation and transduction of human and murine T cells with gammaretroviral vectors were optimized for high-level gene transfer and maximum yield of functional T lymphocytes.
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Newrzela, S., Gunda, B., Laer, D.v. (2009). T Cell Culture for Gammaretroviral Transfer. In: Baum, C. (eds) Genetic Modification of Hematopoietic Stem Cells. Methods In Molecular Biology™, vol 506. Humana Press. https://doi.org/10.1007/978-1-59745-409-4_6
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DOI: https://doi.org/10.1007/978-1-59745-409-4_6
Publisher Name: Humana Press
Print ISBN: 978-1-58829-980-2
Online ISBN: 978-1-59745-409-4
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