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Genetic Modification of Hematopoietic Stem Cells

Methods and Protocols

  • Christopher Baum

Part of the Methods In Molecular Biology™ book series (MIMB, volume 506)

Table of contents

  1. Front Matter
    Pages i-xxi
  2. Guillermo Guenechea, Jose C. Segovia, Juan A. Bueren
    Pages 1-11
  3. Yuk Yin Ng, Miranda R.M. Baert, Edwin F.E. de Haas, Karin Pike-Overzet, Frank J.T. Staal
    Pages 13-21
  4. Ute Modlich, Axel Schambach, Zhixiong Li, Bernhard Schiedlmeier
    Pages 23-31
  5. Francesca Santoni de Sio, Luigi Naldini
    Pages 59-70
  6. Sebastian Newrzela, Brandenburg Gunda, Dorothee von Laer
    Pages 71-82
  7. James Lee, Michel Sadelain, Renier Brentjens
    Pages 83-96
  8. Els Verhoeyen, Caroline Costa, Francois-Loic Cosset
    Pages 97-114
  9. Xin Huang, Andrew Wilber, R. Scott McIvor, Xianzheng Zhou
    Pages 115-126
  10. Evren Alici, Tolga Sutlu, M. Sirac Dilber
    Pages 127-137
  11. Oumeya Adjali, Amélie Montel-Hagen, Louise Swainson, Sophie Marty, Rita Vicente, Cedric Mongellaz et al.
    Pages 171-190
  12. Axel Schambach, William P. Swaney, Johannes C.M. van der Loo
    Pages 191-205
  13. Michaela Scherr, Letizia Venturini, Matthias Eder
    Pages 207-219
  14. Lakshmi Sastry, Kenneth Cornetta
    Pages 243-263
  15. Diana Nordling, Anne Kaiser, Lilith Reeves
    Pages 265-279
  16. Todd Schuesler, Lilith Reeves, Christof von Kalle, Elke Grassman
    Pages 281-298
  17. Zhixiong Li, Ute Modlich, Mishra Anjali
    Pages 311-329
  18. Brian C. Beard, Hans-Peter Kiem
    Pages 341-361
  19. Manfred Schmidt, Kerstin Schwarzwaelder, Cynthia C. Bartholomae, Hanno Glimm, Christof von Kalle
    Pages 363-372
  20. Olga S. Kustikova, Ute Modlich, Boris Fehse
    Pages 373-390
  21. Alessandra Recchia, Fulvio Mavilio
    Pages 391-401
  22. Karin Pike-Overzet, Dick de Ridder, Tom Schonewille, Frank J.T. Staal
    Pages 403-421
  23. Hartmut Geiger, Schleimer David, Kalpana J. Nattamai, Vijg Jan
    Pages 423-436
  24. Eva M. Weissinger, Petra Zürbig, Arnold Ganser
    Pages 437-452
  25. Cornelia Rudolph, Brigitte Schlegelberger
    Pages 453-466
  26. Brenden Balcik, Elke Grassman, Lilith Reeves
    Pages 467-476
  27. Back Matter
    Pages 489-494

About this book

Introduction

With the incredible potential of gene transfer into hematopoietic stem cells, active research in this field has become critically important. In Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols, leading scientists in the field provide a compendium of protocols which cover the subject comprehensively, from the purification and culture of various types of hematopoietic cells for subsequent genetic modification by vector development and technical issues of small and large scale vector production, to the complex issue of monitoring and biosafety studies related to gene-modified hematopoiesis. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which allow the experts to highlight tips on troubleshooting and avoiding known pitfalls.

Unique and cutting-edge, Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols is an ideal, thorough resource to promote further research and the implementation of investigator-driven clinical studies using gene-modified hematopoietic cells.

Keywords

Complex nucleic acids DNA Gene medicine Gene transfer Hematology Hematopoiesis Microarray Mutagenesis PCR Proteomics Termination gene expression gene therapy genes hybridization

Editors and affiliations

  • Christopher Baum
    • 1
  1. 1.Department of Experimental HematologyHannover Medical SchoolHannoverGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-409-4
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-980-2
  • Online ISBN 978-1-59745-409-4
  • Series Print ISSN 1064-3745
  • Buy this book on publisher's site
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