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Isolation of JFH-1 Strain and Development of an HCV Infection System

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Hepatitis C

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 510))

Abstract

Detailed analysis of hepatitis C virus (HCV) has been hampered by the lack of an appropriate viral culture system and small animal models of infection. My group and others have recently reported the production of infectious virus after full-length HCV RNA transfection into Huh-7 cells. This system depends primarily on isolation of a JFH-1 strain from a patient with fulminant hepatitis. The JFH-1 strain belongs to genotype 2a and has high colony-formation efficiency when tested with a subgenomic replicon system. Here, I describe various protocols for isolation of the JFH-1 strain and construction of the HCV infection system. The HCV infection system contributes to our understanding of HCV virology and may permit development of novel antiviral strategies.

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Acknowledgements

This work was partially supported by a grant-in-aid for Scientific Research from the Japan Society for the Promotion of Science, from the Ministry of Health, Labour and Welfare of Japan, and from the Ministry of Education, Culture, Sports, Science and Technology and by the Research on Health Sciences Focusing on Drug Innovation from the Japan Health Sciences Foundation.

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Authors and Affiliations

  1. Second Department of Virology, National Institute of Infectious Diseases, Tokyo, Japan

    Takaji Wakita

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  1. Takaji Wakita
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Editors and Affiliations

  1. Florida State University, Tallahassee, FL, USA

    Hengli Tang

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Wakita, T. (2009). Isolation of JFH-1 Strain and Development of an HCV Infection System. In: Tang, H. (eds) Hepatitis C. Methods in Molecular Biology™, vol 510. Humana Press. https://doi.org/10.1007/978-1-59745-394-3_23

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  • DOI: https://doi.org/10.1007/978-1-59745-394-3_23

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-970-3

  • Online ISBN: 978-1-59745-394-3

  • eBook Packages: Springer Protocols

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