Hepatitis C

Methods and Protocols

  • Hengli Tang

Part of the Methods in Molecular Biology™ book series (MIMB, volume 510)

Table of contents

  1. Front Matter
    Pages i-xxi
  2. Detection and Quantification of HCV

    1. Front Matter
      Pages 1-1
    2. Helene Le Guillou-Guillemette, Francoise Lunel-Fabiani
      Pages 3-14
    3. Filippo Ansaldi, Giancarlo Icardi
      Pages 15-23
    4. Oivier Galy, Isabelle Vincent, Philippe Chevallier, Nicole Lyandrat, Isabelle Chemin
      Pages 25-30
  3. HCV Nomenclature, Genotyping, and Quasispecies

    1. Front Matter
      Pages 31-31
    2. Carla Kuiken, Peter Simmonds
      Pages 33-53
    3. Kathryn J. Rolfe, Tim G. Wreghitt, Graeme J. M. Alexander, Martin D. Curran
      Pages 55-71
  4. HCV Replication in Vitro

    1. Front Matter
      Pages 83-83
    2. Joseph Marcotrigiano, Timothy Tellinghuisen
      Pages 85-94
    3. Petra Neddermann
      Pages 95-110
    4. Nathalie Saint, Roland Montserret, Christophe Chipot, François Penin
      Pages 125-143
    5. Volker Lohmann
      Pages 145-163
    6. Timothy L. Tellinghuisen, Brett D. Lindenbach
      Pages 165-175
    7. Wengang Yang, Mingjun Huang
      Pages 177-184
    8. Kyungsoo Chang, Tianyi Wang, Guangxiang Luo
      Pages 185-193
    9. Volker Brass, Rainer Gosert, Darius Moradpour
      Pages 195-209

About this book


With the development of robust cell-culturing systems capable of Hepatitis C Virus replication and infection, the past decade has witnessed an explosion of innovative research directed at understanding HCV biology in the context of the authentic replicative cycle of the virus. By providing a compilation of cutting-edge research techniques currently in use in HCV labs worldwide, Hepatitis C: Methods and Protocols, Second Edition fully updates the previous edition to reflect this progress which promises to unlock the remaining vital information regarding this human pathogen and its interactions. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Fully updated and comprehensive, Hepatitis C: Methods and Protocols, Second Edition serves as a valuable reference guide for basic and clinical researchers using HCV as a model and striving to better understand this critical disease.


Antigen Genotypes HCV research Hepatitis Quasispecies RNA replication Virus infection infectious

Editors and affiliations

  • Hengli Tang
    • 1
  1. 1.Florida State UniversityTallahasseeUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-394-3
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-970-3
  • Online ISBN 978-1-59745-394-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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