Abstract
Lyophilization, the removal of water by freezing and then volatilization at low pressure and temperature, has been employed as a standard long-term preservation method for many filamentous fungi. The method outlined involves the use of standard shelf freeze-drying and skimmed milk as a suspending solution/lyoprotectant. This approach has been employed to freeze-dry the majority of the 50,000 fungal strains that have been successfully lyophilized at the Centraal bureau voor Schimmelcultures (CBS) culture collection (http://www.cbs.knaw.nl/).
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© 2007 Humana Press Inc., Totowa, NJ
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Tan, C.Sh., van Ingen, C.W., Stalpers, J.A. (2007). Freeze-Drying Fungi Using a Shelf-Freeze Drier. In: Day, J.G., Stacey, G.N. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology™, vol 368. Humana Press. https://doi.org/10.1007/978-1-59745-362-2_8
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DOI: https://doi.org/10.1007/978-1-59745-362-2_8
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