Abstract
Immunohistochemistry is widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions and is an exceptionally powerful method to demonstrate the localization of cellular components. Immunoglobulins (antibodies) are glycoproteins and are divided into five major classes. IgG, which composes approximately 75% of the immunoglobulins in human serum, is most commonly used for immunostaining. Two types of detection systems, fluorescent and enzyme based are used for immunostaining. The choice of detection system depends on the type of sample and the availability of fluorescent or bight field microscopes as well as the type of information the investigator would like to obtain. This chapter provides an overview of antibody characteristics, and their use in immunostaining.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Polak JM, VanNoorden S (2003) Introduction to Immunocytochemistry. BIOS Scientific Publishers, Oxford, UK 196 pp
Beesely J (1993) Immunocytochemistry: a practical approach. Oxford University Press, UK, p 276
Larson L-I (1988) Immunocytochemistry: theory and practice. CRC Press, Boca Raton, FL 298 pp
Sternberger LA (1979) Immunocytochemistry, 2nd edn. Wiley, New York
Hamilton RG (2001) The human IgG subclasses. Mohan C. (ed) Calbiochem-Novabiochem http: www.emdbiosciences.com/docs/docs/LIT/CB0051.pdf
Coons AH, Creech HJ, Jones RN (1941) Immunological properties of an antibody containing a fluorescent group. Proc Soc Exp Biol Med 47:200–202
Nakane P, Pierce G (1966) Enzyme-labeled antibodies: preparation and application for the localization of antigens. J Histochem Cytochem 14:929–931
Farr A, Nakane P (1981) Immunohistochemistry with enzyme labeled antibodies: a brief review. J Immunol Methods 47:129–144
Sternberger LA, Sternberger NH (1986) The unlabeled antibody method: comparison of peroxidase-antiperoxidase with avidin–biotin complex by a new method of quantification. J Histochem Cytochem 34:599–605
Coons AH, Leduc EH, Connolly JM (1955) Studies on antibody production I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J Exp Med 102:49–60
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Oliver, C., Jamur, M.C. (2010). Overview of Antibodies for Immunochemistry. In: Oliver, C., Jamur, M. (eds) Immunocytochemical Methods and Protocols. Methods in Molecular Biology, vol 588. Humana Press. https://doi.org/10.1007/978-1-59745-324-0_1
Download citation
DOI: https://doi.org/10.1007/978-1-59745-324-0_1
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-58829-463-0
Online ISBN: 978-1-59745-324-0
eBook Packages: Springer Protocols