Immunocytochemical Methods and Protocols

  • Constance Oliver
  • Maria Célia Jamur

Part of the Methods in Molecular Biology book series (MIMB, volume 588)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Antibody Preparation

    1. Front Matter
      Pages 1-1
    2. Constance Oliver, Maria Célia Jamur
      Pages 3-9
    3. Ana Cristina Grodzki, Elsa Berenstein
      Pages 11-13
    4. Ana Cristina Grodzki, Elsa Berenstein
      Pages 15-26
    5. Ana Cristina Grodzki, Elsa Berenstein
      Pages 27-32
    6. Su-Yau Mao, J. Michael Mullins
      Pages 43-48
    7. Su-Yau Mao
      Pages 49-52
  3. Tissue Preparation for Light Microscopic Analysis

    1. Front Matter
      Pages 54-54
    2. Maria Célia Jamur, Constance Oliver
      Pages 55-61
    3. Maria Célia Jamur, Constance Oliver
      Pages 63-66
    4. Gary L. Bratthauer
      Pages 67-73
    5. Gary L. Bratthauer
      Pages 75-84
    6. Gary L. Bratthauer
      Pages 85-92
    7. Gary L. Bratthauer
      Pages 93-102
    8. Laszlo Krenacs, Tibor Krenacs, Eva Stelkovics, Mark Raffeld
      Pages 103-119
  4. Bright Field Detection Systems

    1. Front Matter
      Pages 122-122
    2. J. Michael Mullins
      Pages 123-134
    3. Maria Veronica Dávila Pástor
      Pages 135-142

About this book

Introduction

The landscape of immunocytochemistry has significantly evolved in past years. Once a laborious technique with limited application, the introduction of new detection systems and instrumentation has facilitated the widespread use of immunocytochemical methods. In the post-genomic era, the use of immunocytochemical methods has grown to include the identification of a broad spectrum of antigens including proteins, carbohydrates and lipids from virtually every organism. In Immunocytochemical Methods and Protocols, Third Edition, expert researchers explore the modern methods employed in the field today,  now widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions, and to demonstrate the localization of subcellular components. Chapters provide methods that are designed to be general in their application and to appeal to investigators across all disciplines, including those in research and clinical settings. Composed in the highly successful Methods in Molecular Biology™ series format, chapters contain a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

 Comprehensive and ground-breaking,  Immunocytochemical Methods and Protocols, Third Edition, brings together a variety of methods that are normally limited to applications in a specific area, providing a unique and vital guide for researchers and clinicians alike.

Keywords

Antibody Antigen Fluorescence microscopy IgG Immunohistochemistry Immunostaining

Editors and affiliations

  • Constance Oliver
    • 1
  • Maria Célia Jamur
    • 2
  1. 1.Fac. Medicina de Ribeirão PretoUniversidade de São PauloRibeirão PretoBrazil
  2. 2.Fac. Medicina de Ribeirão PretoUniversidade de São PauloRibeirão PretoBrazil

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-324-0
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-463-0
  • Online ISBN 978-1-59745-324-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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