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Improving Marginal Crystals

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Macromolecular Crystallography Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 363))

Abstract

The physical chemistry of crystal growth can help to identify directions in which to look for improved crystal properties. In this chapter, we summarize how crystal growth depends on parameters that can be controlled experimentally, and relate them to the tools available for optimizing a particular crystal form for crystal shape, volume, and diffraction quality. Our purpose is to sketch the conceptual basis of optimization and to provide sample protocols derived from those foundations. We hope to assist even those who chose not to use systematic methods by enabling them to carry out rudimentary optimization searches armed with a better understanding of how the underlying physical chemistry operates.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC

    Charles W. Carter Jr.

  2. Laboratoire de Cristallographie et RMN Biologiques, Université René Descartes, Paris, France

    Madeleine Riès-Kautt

Authors
  1. Charles W. Carter Jr.
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  2. Madeleine Riès-Kautt
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Editor information

Editors and Affiliations

  1. Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT

    Sylvie Doublié

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© 2007 Humana Press Inc.

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Carter, C.W., Riès-Kautt, M. (2007). Improving Marginal Crystals. In: Walker, J.M., Doublié, S. (eds) Macromolecular Crystallography Protocols. Methods in Molecular Biology, vol 363. Humana Press. https://doi.org/10.1007/978-1-59745-209-0_8

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  • DOI: https://doi.org/10.1007/978-1-59745-209-0_8

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-292-6

  • Online ISBN: 978-1-59745-209-0

  • eBook Packages: Springer Protocols

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