Macromolecular Crystallography Protocols

Volume 1, Preparation and Crystallization of Macromolecules

  • John M. Walker
  • Sylvie Doublié

Part of the Methods in Molecular Biology book series (MIMB, volume 363)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Joseph E. Tropea, Scott Cherry, Sreedevi Nallamsetty, Christophe Bignon, David S. Waugh
    Pages 1-19
  3. Sophie Quevillon-Cheruel, Bruno Collinet, Lionel Trésaugues, Philippe Minard, Gilles Henckes, Robert Aufrère et al.
    Pages 21-37
  4. Sonia Longhi, François Ferron, Marie-Pierre Egloff
    Pages 59-90
  5. Charles W. Carter Jr., Madeleine Riès-Kautt
    Pages 153-174
  6. Thomas Hollis
    Pages 225-237
  7. Barbara L. Golden
    Pages 239-257
  8. Eiji Obayashi, Chris Oubridge, Daniel Pomeranz Krummel, Kiyoshi Nagai
    Pages 259-276
  9. Back Matter
    Pages 277-279

About this book

Introduction

Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1, Preparation and Crystallization of Macromolecules and Volume 2, Structure Determination, explore recent advances that have accelerated the pace of structural determination and made crystallography accessible to a broader range of investigators.

Volume 1 is composed of detailed protocols for the preparation and optimization of crystals, including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization, as well as computational techniques for data collection, phasing, and refinement. The volume concludes with a detailed and insightful survey of available crystallographic software. These volumes will be an indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure.

Keywords

Endoplasmatisches Reticulum Polyacrylamid-Gelelektrophorese Termination escherichia coli membrane proteins protein complexes proteins

Editors and affiliations

  • John M. Walker
  • Sylvie Doublié
    • 1
  1. 1.Department of Microbiology and Molecular GeneticsUniversity of VermontBurlington

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-209-0
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-292-6
  • Online ISBN 978-1-59745-209-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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