Summary
A novel in vitro method is described wherein gene expression profiling is reflective and informative for the way how syncytiotrophoblast cells shed RNA products in vivo in maternal plasma. After controlled denudation, RNA is obtained selectively from the syncytiotrophoblast cells of trisomy 21 placentae. cDNA copies are subsequently analyzed by microarray profiling and cDNA cloning with sequencing. Given the preponderance of 5′ mRNA fragments lacking a poly-A tail, the placental RNA products are amplified after polymerase A-mediated tailing by using a method originally designed for small-sized microRNAs. This approach, when combined with cDNA library or microarray expression screening, is a novel in vitro method to screen for syncytiotrophoblast-derived RNA products representative of trisomy 21 placental RNA as present in vivo in maternal plasma.
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Acknowledgement
M.v.D. is supported by the SAFE Network Project LSHB-CT-2004-503243).
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Go, A.T. et al. (2008). A Novel Method to Identify Syncytiotrophoblast-Derived RNA Products Representative of Trisomy 21 Placental RNA in Maternal Plasma. In: Hahn, S., Jackson, L.G. (eds) Prenatal Diagnosis. Methods in Molecular Biology™, vol 444. Humana Press. https://doi.org/10.1007/978-1-59745-066-9_23
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DOI: https://doi.org/10.1007/978-1-59745-066-9_23
Publisher Name: Humana Press
Print ISBN: 978-1-58829-803-4
Online ISBN: 978-1-59745-066-9
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