Abstract
Uncomplicated urinary tract infections, especially those caused by Escherichia coli, have historically been widely studied. However, complicated urinary tract infections are presenting ever increasing healthcare challenges, particularly with Proteus mirabilis. P. mirabilis is often found on indwelling urinary catheters causing monomicrobial and polymicrobial catheter-associated urinary tract infection (CAUTI). Widespread antibiotic resistance, combined with the ability of P. mirabilis to form urinary calculi during infection, warrants further investigation of this pathogen and its host interaction in an infection model that more closely mimics the presence of an indwelling urinary catheter. Here, we describe the methods necessary to establish a murine model of P. mirabilis CAUTI.
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Acknowledgments
The authors would like to thank Harry Mobley and members of the Mobley laboratory for their helpful comments and critiques during the initial development of this protocol. This work was supported by the National Institute of Diabetes Digestive and Kidney Disorders (R00 DK105205 to C.E.A.).
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Smith, S.N., Armbruster, C.E. (2019). Indwelling Urinary Catheter Model of Proteus mirabilis Infection. In: Pearson, M. (eds) Proteus mirabilis. Methods in Molecular Biology, vol 2021. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9601-8_17
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DOI: https://doi.org/10.1007/978-1-4939-9601-8_17
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Publisher Name: Humana, New York, NY
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