Proteus mirabilis

1. Front Matter

   Pages i-x

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2. Proteus mirabilis Overview

   • Harry L. T. Mobley

   Pages 1-4

3. Culture Methods for Proteus mirabilis

   • Melanie M. Pearson

   Pages 5-13

4. Methods for Studying Swarming and Swimming Motility

   • Melanie M. Pearson

   Pages 15-25

5. Testing the Ability of Compounds to Induce Swarming

   • Chelsie E. Armbruster

   Pages 27-34

6. Purification of Native Flagellin

   • María José González, Victoria Iribarnegaray, Pablo Zunino, Paola Scavone

   Pages 35-44

7. Analysis of Proteus mirabilis Social Behaviors on Surfaces

   • Kristin Little, Karine A. Gibbs

   Pages 45-59

8. Insertional Mutagenesis Protocol for Constructing Single or Sequential Mutations

   • Melanie M. Pearson, Stephanie D. Himpsl, Harry L. T. Mobley

   Pages 61-76

9. Allelic Exchange Mutagenesis in Proteus mirabilis

   • Kristen E. Howery, Philip N. Rather

   Pages 77-84

10. Quantification of Urease Activity

    • Shawn Richmond, Alejandra Yep

    Pages 85-96

11. Siderophore Detection Using Chrome Azurol S and Cross-Feeding Assays

    • Stephanie D. Himpsl, Harry L. T. Mobley

    Pages 97-108

12. Using Hemagglutination, Surface Shearing, and Acid Treatment to Study Fimbriae in Proteus mirabilis

    • Stephanie D. Himpsl, Melanie M. Pearson, Harry L. T. Mobley

    Pages 109-120

13. Phase Variation of the mrp Fimbrial Promoter

    • Melanie M. Pearson

    Pages 121-127

14. Adherence of Proteus mirabilis to Uroepithelial Cells

    • María José González, Victoria Iribarnegaray, Paola Scavone, Pablo Zunino

    Pages 129-137

15. An In Vitro Bladder Model for Studying Catheter-Associated Urinary Tract Infection and Associated Analysis of Biofilms

    • Jonathan Nzakizwanayo, Harriet Pelling, Scarlet Milo, Brian V. Jones

    Pages 139-158

16. Independent Transurethral Urinary Tract Inoculation in a Murine Model of Ascending Infection with Proteus mirabilis

    • Sara N. Smith

    Pages 159-172

17. Cochallenge Inoculation with Proteus mirabilis in a Murine Transurethral Urinary Tract Model of Ascending Infection

    • Sara N. Smith

    Pages 173-186

18. Indwelling Urinary Catheter Model of Proteus mirabilis Infection

    • Sara N. Smith, Chelsie E. Armbruster

    Pages 187-200

19. Vaccination to Protect Against Proteus mirabilis Challenge Utilizing the Ascending Model of Urinary Tract Infection

    • Sara N. Smith, Stephanie D. Himpsl, Harry L. T. Mobley

    Pages 201-215

20. Characterization of Proteus mirabilis Lipopolysaccharide Samples by Infrared Spectroscopy and Serological Methods

    • Katarzyna Durlik, Grzegorz Czerwonka, Paulina Żarnowiec, Wiesław Kaca

    Pages 217-230

This detailed volume explores essential protocols for the study of Proteus mirabilis which, despite its genetic relatedness to species such as E. coli, often requires specialized handling techniques. This opportunistic bacterial pathogen, most often known as a causative agent of complicated urinary tract infection, is addressed in chapters from global experts in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Proteus mirabilis: Methods and Protocols serves as an ideal guide for researchers intrigued by the renewed appreciation for the medical impact and environmental adaptability of this organism, coupled with continued fascination for its dynamic behavior.

• P. mirabilis
• Bacterium
• Bacterial pathogen
• Urinary tract infections
• Swarming motility
• Environmental adaptability

• Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, USA

  Melanie M. Pearson

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