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Assessment of fHbp Expression Level by Reverse Transcriptase Quantitative PCR and Promoter Sequence Analysis

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Neisseria meningitidis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1969))

Abstract

Reverse transcriptase quantitative PCR (RT qPCR) is widely used for assessing the levels of expression of specific genes in various organisms. Here we describe a RT qPCR assay designed to determine the level of expression of fHbp in multiple isolates of Neisseria meningitidis. The level of expression is measured by a two-step qPCR and is associated with a promoter region analysis.

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Author information

Authors and Affiliations

  1. Department of Genetics and Genome Biology, University of Leicester, Leicester, UK

    Caroline Cayrou & Christopher D. Bayliss

Authors
  1. Caroline Cayrou
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  2. Christopher D. Bayliss
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Corresponding author

Correspondence to Christopher D. Bayliss .

Editor information

Editors and Affiliations

  1. Institute For Glycomics, Griffith University, Southport, QLD, Australia

    Kate L. Seib

  2. Institute For Glycomics, Griffith University, Southport, QLD, Australia

    Ian R. Peak

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Cayrou, C., Bayliss, C.D. (2019). Assessment of fHbp Expression Level by Reverse Transcriptase Quantitative PCR and Promoter Sequence Analysis. In: Seib, K., Peak, I. (eds) Neisseria meningitidis. Methods in Molecular Biology, vol 1969. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9202-7_16

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  • DOI: https://doi.org/10.1007/978-1-4939-9202-7_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9201-0

  • Online ISBN: 978-1-4939-9202-7

  • eBook Packages: Springer Protocols

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