Methods of Isolation and Analysis of TREG Immune Infiltrates from Injured and Dystrophic Skeletal Muscle

  • Dalia Burzyn
  • Amy Wagers
  • Diane Mathis
  • Massimiliano CerlettiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1899)


The immune infiltrate present in acutely injured or dystrophic skeletal muscle has been shown to play an important role in the process of muscle regeneration. Our work has described, for the first time, muscle regulatory T cells (Tregs), a unique population in phenotype and function capable of promoting skeletal muscle repair. Herein, we describe the methods we have optimized to study muscle Tregs, including their isolation from injured muscle, immuno-labeling for analysis/separation by flow cytometry, and measurement of their proliferation status.

Key words

Regulatory T cell Skeletal muscle infiltrate Tissue-resident leukocytes Treg proliferation Skeletal muscle injury Muscular dystrophy 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Dalia Burzyn
    • 1
  • Amy Wagers
    • 2
  • Diane Mathis
    • 1
  • Massimiliano Cerletti
    • 3
    Email author
  1. 1.Division of Immunology, Department of Microbiology and ImmunobiologyHarvard Medical SchoolBostonUSA
  2. 2.Department of Stem Cell and Regenerative BiologyHarvard Stem Cell Institute, Harvard UniversityCambridgeUSA
  3. 3.UCL Research Department for Surgical Biotechnologies & Institute for Immunity and TransplantationUniversity College LondonLondonUK

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