Abstract
Adipose-derived stromal cells (ASCs) are a promising population of cells that may be useful for the regeneration of human tissue defects. ASCs are capable of forming bone tissue in vitro and in vivo. Further work is required to determine the optimal conditions that will allow human ASCs to regenerate tissue in clinically significant tissue defects. Here we present three experimental protocols that are indispensable for the study of ASC osteogenic activity.
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References
de Boer HH (1988) The history of bone grafts. Clin Orthop Relat Res 226:292–298
Kolk A, Handschel J, Drescher W, Rothamel D, Kloss F, Blessmann M, Heiland M, Wolff KD, Smeets R (2012) Current trends and future perspectives of bone substitute materials—from space holders to innovative biomaterials. J Craniomaxillofac Surg 40(8):706–718. https://doi.org/10.1016/j.jcms.2012.01.002
Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, Alfonso ZC, Fraser JK, Benhaim P, Hedrick MH (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13(12):4279–4295. https://doi.org/10.1091/mbc.E02-02-0105
Casteilla L, Planat-Benard V, Laharrague P, Cousin B (2011) Adipose-derived stromal cells: their identity and uses in clinical trials, an update. World J Stem Cells 3(4):25–33. https://doi.org/10.4252/wjsc.v3.i4.25
Gimble JM, Bunnell BA, Frazier T, Rowan B, Shah F, Thomas-Porch C, Wu X (2013) Adipose-derived stromal/stem cells: a primer. Organogenesis 9(1):3–10. https://doi.org/10.4161/org.24279
Bourin P, Bunnell BA, Casteilla L, Dominici M, Katz AJ, March KL, Redl H, Rubin JP, Yoshimura K, Gimble JM (2013) Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT). Cytotherapy 15(6):641–648. https://doi.org/10.1016/j.jcyt.2013.02.006
Zielins ER, Luan A, Brett EA, Longaker MT, Wan DC (2015) Therapeutic applications of human adipose-derived stromal cells for soft tissue reconstruction. Discov Med 19(105):245–253
Lo DD, Hyun JS, Chung MT, Montoro DT, Zimmermann A, Grova MM, Lee M, Wan DC, Longaker MT (2012) Repair of a critical-sized calvarial defect model using adipose-derived stromal cells harvested from lipoaspirate. J Vis Exp (68). https://doi.org/10.3791/4221
Sanchez-Duffhues G, Hiepen C, Knaus P, Ten Dijke P (2015) Bone morphogenetic protein signaling in bone homeostasis. Bone 80:43–59. https://doi.org/10.1016/j.bone.2015.05.025
Fan J, Im CS, Guo M, Cui ZK, Fartash A, Kim S, Patel N, Bezouglaia O, Wu BM, Wang CY, Aghaloo TL, Lee M (2016) Enhanced osteogenesis of adipose-derived stem cells by regulating bone morphogenetic protein signaling antagonists and agonists. Stem Cells Transl Med 5(4):539–551. https://doi.org/10.5966/sctm.2015-0249
Walmsley GG, Rinkevich Y, Hu MS, Montoro DT, Lo DD, McArdle A, Maan ZN, Morrison SD, Duscher D, Whittam AJ, Wong VW, Weissman IL, Gurtner GC, Longaker MT (2015) Live fibroblast harvest reveals surface marker shift in vitro. Tissue Eng C Methods 21(3):314–321. https://doi.org/10.1089/ten.TEC.2014.0118
Chung MT, Zimmermann AS, Paik KJ, Morrison SD, Hyun JS, Lo DD, McArdle A, Montoro DT, Walmsley GG, Senarath-Yapa K, Sorkin M, Rennert R, Chen HH, Chung AS, Vistnes D, Gurtner GC, Longaker MT, Wan DC (2013) Isolation of human adipose-derived stromal cells using laser-assisted liposuction and their therapeutic potential in regenerative medicine. Stem Cells Transl Med 2(10):808–817. https://doi.org/10.5966/sctm.2012-0183
Tevlin R, McArdle A, Brett E, Chung MT, Paik K, Seo EY, Walmsley GG, Duldulao CR, Atashroo D, Zielins E, Vistnes S, Chan CK, Wan DC, Longaker MT (2016) A novel method of human adipose-derived stem cell isolation with resultant increased cell yield. Plast Reconstr Surg 138(6):983e–996e. https://doi.org/10.1097/PRS.0000000000002790
Dawson AB (1926) A note on the staining of the skeleton of cleared specimens with alizarin red S. Stain Technol 1(4):123–123
Gentile P, Chiono V, Carmagnola I, Hatton PV (2014) An overview of poly(lactic-co-glycolic) acid (PLGA)-based biomaterials for bone tissue engineering. Int J Mol Sci 15(3):3640–3659. https://doi.org/10.3390/ijms15033640
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Marshall, C.D., Brett, E.A., Moore, A.L., Wan, D.C., Longaker, M.T. (2019). In Vitro and In Vivo Osteogenic Differentiation of Human Adipose-Derived Stromal Cells. In: Rogers, M. (eds) Bone Morphogenetic Proteins. Methods in Molecular Biology, vol 1891. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8904-1_2
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DOI: https://doi.org/10.1007/978-1-4939-8904-1_2
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