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Bone Morphogenetic Proteins

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Melissa B. Rogers

    1. New Jersey Medical School, Rutgers Biomedical and Health Sciences, Newark, USA

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provide step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1891)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Common Cell Lines Used to Study Bone Morphogenetic Proteins (BMPs)

    • Jessica Ann Cottrell, Donya Burgess, Sarah Michaels, Melissa B. Rogers
    Pages 1-8

  3. In Vitro and In Vivo Osteogenic Differentiation of Human Adipose-Derived Stromal Cells

    • Clement D. Marshall, Elizabeth A. Brett, Alessandra L. Moore, Derrick C. Wan, Michael T. Longaker
    Pages 9-18

  4. Cell-Based Gene Therapy System for Delivering BMPs

    • Austin Dickerson, Eleanor L. Davis, Corinne Sonnet, Alan R. Davis, Elizabeth A. Olmsted-Davis
    Pages 19-28

  5. Generation of Endogenous BMP Transcriptional Reporter Cells Through CRISPR/Cas9 Genome Editing

    • Luke D. Hutchinson, Polyxeni Bozatzi, Thomas Macartney, Gopal P. Sapkota
    Pages 29-35

  6. High-Throughput, Biosensor-Based Approach to Examine Bone Morphogenetic Protein (BMP)–Receptor Interactions

    • Senem Aykul, Erik Martinez-Hackert
    Pages 37-49

  7. Mutagenesis and Imaging Studies of BMP Signaling Mechanisms in C. elegans

    • Cathy Savage-Dunn, Ryan J. Gleason, Jun Liu, Richard W. Padgett
    Pages 51-73

  8. Gene Regulation of BMP Ligands in Drosophila

    • Brian G. Stultz, Deborah A. Hursh
    Pages 75-89

  9. Using Amphioxus as a Basal Chordate Model to Study BMP Signaling Pathway

    • Luok Wen Yong, Iryna Kozmikova, Jr-Kai Yu
    Pages 91-114

  10. Proteolytic Activation of Bmps: Analysis of Cleavage in Xenopus Oocytes and Embryos

    • Hyung-Seok Kim, Autumn McKnite, Jan L. Christian
    Pages 115-133

  11. Imaging and Quantification of P-Smad1/5 in Zebrafish Blastula and Gastrula Embryos

    • Joseph Zinski, Francesca Tuazon, Yan Huang, Mary Mullins, David Umulis
    Pages 135-154

  12. An Adult Zebrafish Model of Fibrodysplasia Ossificans Progressiva

    • Melissa LaBonty, Pamela C. Yelick
    Pages 155-163

  13. Generation and Identification of Genetically Modified Mice for BMP Receptors

    • Jingwen Yang, Yuji Mishina
    Pages 165-177

  14. Phenotypic Analyses of Genetically Modified Mice for BMP Receptors

    • Jingwen Yang, Yuji Mishina
    Pages 179-189

  15. Immunofluorescent Visualization of BMP Signaling Activation on Paraffin-Embedded Tissue Sections

    • Maaike Alkema, Marie-José Goumans, Boudewijn P. T. Kruithof
    Pages 191-200

  16. Spatial and Quantitative Detection of BMP Activity in Mouse Embryonic Limb Buds

    • Marcelo Rocha Marques, Jean-Denis Bénazet
    Pages 201-219

  17. Pharmacologic Strategies for Assaying BMP Signaling Function

    • Teresa Dinter, Geoffrey A. Bocobo, Paul B. Yu
    Pages 221-233

  18. Bone Morphogenetic Proteins (BMPs) and Bone Regeneration

    • Deboleena Kanjilal, Jessica Ann Cottrell
    Pages 235-245

  19. Heterotopic Ossification in Mouse Models of Fibrodysplasia Ossificans Progressiva

    • Salin A. Chakkalakal, Eileen M. Shore
    Pages 247-255

  20. Double-Humanized Mouse Model to Study Bone Morphogenetic Protein (BMP) Signaling in Tumor Xenografts

    • Jenna Newman, Rachel NeMoyer, Andrew Zloza, John Langenfeld
    Pages 257-262
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Keywords

About this book

This volume provides methods to study bone morphogenetic proteins (BMPs) and BMP modulators in cell culture, in both invertebrate and vertebrate animal models, and for therapeutic applications. Chapters guide the reader in the use of primary and immortalized cell lines, methodologies that exploit transcription and receptor mechanisms, newly developed animal models using nematodes, flies, amphioxus, frogs, zebrafish, and genetically engineered mice, and pre-clinical approaches to understanding BMP function in bone regeneration, heterotopic ossification, and cancers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

  Authoritative and cutting-edge, Bone Morphogenetic Proteins: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • New Jersey Medical School, Rutgers Biomedical and Health Sciences, Newark, USA

    Melissa B. Rogers

Bibliographic Information

  • Book Title: Bone Morphogenetic Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Melissa B. Rogers

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8904-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8903-4Published: 10 November 2018

  • eBook ISBN: 978-1-4939-8904-1Published: 09 November 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 265

  • Number of Illustrations: 10 b/w illustrations, 27 illustrations in colour

  • Topics: Molecular Medicine

  • Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Health & Hospitals, Pharma

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