Abstract
Correlative live-cell imaging and super-resolution microscopy of autophagy was developed to combine the temporal resolution of time-lapse fluorescence microscopy with the spatial resolution of super-resolution microscopy. HEK293 cells that express recombinant proteins of interest fused to fluorescent tags are imaged live to capture the formation of autophagosomes, fixed on stage to “snap-freeze” these structures, stained with appropriate antibodies, relocated, and imaged at super resolution by direct stochastic optical reconstruction microscopy. This chapter provides an easy-to-follow protocol along with practical tips and background information to help set up and perform an experiment.
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Karanasios, E. (2019). Correlative Live-Cell Imaging and Super-Resolution Microscopy of Autophagy. In: Ktistakis, N., Florey, O. (eds) Autophagy. Methods in Molecular Biology, vol 1880. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8873-0_15
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DOI: https://doi.org/10.1007/978-1-4939-8873-0_15
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-8873-0
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