Methods and Protocols

  • Nicholas Ktistakis
  • Oliver Florey

Part of the Methods in Molecular Biology book series (MIMB, volume 1880)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Autophagry In Vitro

    1. Front Matter
      Pages 1-1
    2. Abul K. Tarafder, Audrey Guesdon, Tanja Kuhm, Carsten Sachse
      Pages 3-15
    3. Melanie Schwarten, Oliver H. Weiergräber, Dušan Petrović, Birgit Strodel, Dieter Willbold
      Pages 17-56
    4. Kazuto Ohashi, Chinatsu Otomo, Zoltan Metlagel, Takanori Otomo
      Pages 57-75
    5. Akinori Yamasaki, Yasunori Watanabe, Nobuo N. Noda
      Pages 77-90
    6. Javier H. Hervás, Zuriñe Antón, Alicia Alonso
      Pages 91-117
    7. Peter Mayrhofer, Thomas Wollert
      Pages 119-133
    8. Mads Skytte Rasmussen, Åsa Birna Birgisdottir, Terje Johansen
      Pages 149-161
    9. Jaime Agudo-Canalejo, Roland L. Knorr
      Pages 173-188
    10. Christine Abert, Sascha Martens
      Pages 189-196
  3. Imaging Autophagy in Tissue Culture

    1. Front Matter
      Pages 197-197
    2. Sigurdur Gudmundsson, Jenny Kahlhofer, Nastassia Baylac, Katri Kallio, Eeva-Liisa Eskelinen
      Pages 199-209
    3. Hiroyuki Ueda, Ouin Kunitaki, Maho Hamasaki
      Pages 223-230
    4. Audrey Dong, Vineet Vinay Kulkarni, Sandra Maday
      Pages 243-256
    5. Petros Stathakos, Natalia Jimenez-Moreno, Lucy Crompton, Paul Nistor, Maeve A. Caldwell, Jon D. Lane
      Pages 257-280
    6. Elise Jacquin, Katherine Fletcher, Oliver Florey
      Pages 295-303
  4. Assays to Measure (and Screen For) Autophagy and Related Processes

    1. Front Matter
      Pages 305-305
    2. Nikolai Engedal, Morten Luhr, Paula Szalai, Per O. Seglen
      Pages 307-313
    3. Maria M. Lazari, Idil Orhon, Patrice Codogno, Nicolas Dupont
      Pages 331-340
    4. Maria New, Tim Van Acker, Ming Jiang, Rebecca Saunders, Jaclyn S. Long, Jun-Ichi Sakamaki et al.
      Pages 359-374
    5. Elena Seranova, Carl Ward, Miruna Chipara, Tatiana R. Rosenstock, Sovan Sarkar
      Pages 389-428
    6. Amelie J. Mueller, Tassula Proikas-Cezanne
      Pages 429-445
    7. Jens C. Hamann, Sung Eun Kim, Michael Overholtzer
      Pages 447-454
    8. Monica Loi, Laure-Anne Ligeon, Christian Münz
      Pages 455-477
  5. Measuring and Imaging Autophagy In Vitro

    1. Front Matter
      Pages 479-479
    2. Nazmul Huda, Hui Zou, Shengmin Yan, Bilon Khambu, Xiao-Ming Yin
      Pages 481-489
    3. Carlo Follo, Dario Barbone, William G. Richards, Raphael Bueno, V. Courtney Broaddus
      Pages 491-510
    4. Meenakshi Banerjee, Yunjie Huang, Madhu M. Ouseph, Smita Joshi, Irina Pokrovskaya, Brian Storrie et al.
      Pages 511-528
    5. Xigui Chen, Kanoh Kondo, Hitoshi Okazawa
      Pages 529-534
    6. Takashi Ueno, Masaaki Komatsu
      Pages 535-540
    7. Alejandro Ropolo, Daniel Grasso, Maria I. Vaccaro
      Pages 541-554
    8. Sivan Henis-Korenblit, Alicia Meléndez
      Pages 561-586
  6. Mitophagy and Other Selective Autophagy Pathways

    1. Front Matter
      Pages 587-587
    2. Szabolcs Takáts, Sarolta Tóth, Győző Szenci, Gábor Juhász
      Pages 589-600
    3. Andrew S. Moore, Erika L. F. Holzbaur
      Pages 601-610
    4. Cheng-Wei Hsieh, Wei Yuan Yang
      Pages 611-619
    5. Panagiotis Tsapras, Anne-Claire Jacomin, Ioannis P. Nezis
      Pages 643-653
    6. Konstantinos Palikaras, Eirini Lionaki, Nektarios Tavernarakis
      Pages 655-668
    7. Panagiota Kolitsida, Hagai Abeliovich
      Pages 669-678

About this book


This volume details a comprehensive and extensive set of protocols for the study of autophagy in vitro and in vivo. Chapters focus on mammals, various model organisms, and provide protocols for the study of autophagy-related processes outside of the canonical autophagy pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Autophagy: Methods and Protocols aims to ensure successful results in the further study of this vital field.


Drosophila C. elegans xenophagy mitophagy macropinocytosis

Editors and affiliations

  • Nicholas Ktistakis
    • 1
  • Oliver Florey
    • 2
  1. 1.Signalling ProgrammeBabraham InstituteCambridgeUK
  2. 2.Signalling ProgrammeBabraham InstituteCambridgeUK

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8872-3
  • Online ISBN 978-1-4939-8873-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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