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Dynamic Light Scattering Analysis to Dissect Intermediates of SNARE-Mediated Membrane Fusion

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SNAREs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1860))

Abstract

Dynamic light scattering (DLS) spectroscopy provides rapid information on the size distribution of a large number of particles in a mixture. Vesicle sizes change during the merger of lipid bilayers, and DLS analysis can provide rapid, accurate, and non-perturbative quantification of the size distribution of proteoliposomes in SNARE-dependent membrane fusion. In this chapter, we describe the methodologies and reagents used for DLS spectroscopy in a biochemical and biophysical study of SNARE-mediated membrane fusion.

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Acknowledgments

This work was supported by the National Research Foundation of Korea (NRF) of the Korean Government (2017R1A2B2010292, 2017R1A6A1A03015642, and NRF-2017R1A2B2008211), and the KIST Young Fellow Program.

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Author notes

  1. Byoungjae Kong and Yoosoo Yang are contributed equally to this work.

Authors and Affiliations

  1. Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, Republic of Korea

    Byoungjae Kong & Dae-Hyuk Kweon

  2. Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea

    Yoosoo Yang

  3. Division for Bio-Medical Science and Technology, KIST School, Korea University of Science and Technology, Seoul, Republic of Korea

    Yoosoo Yang

  4. Biomedical Institute for Convergence, Sungkyunkwan University, Suwon, Republic of Korea

    Dae-Hyuk Kweon

Authors
  1. Byoungjae Kong
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  2. Yoosoo Yang
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  3. Dae-Hyuk Kweon
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Corresponding author

Correspondence to Dae-Hyuk Kweon .

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Editors and Affiliations

  1. Department of Biochemistry, University of Illinois Urbana-Champaign, Urbana, IL, USA

    Rutilio Fratti

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Kong, B., Yang, Y., Kweon, DH. (2019). Dynamic Light Scattering Analysis to Dissect Intermediates of SNARE-Mediated Membrane Fusion. In: Fratti, R. (eds) SNAREs. Methods in Molecular Biology, vol 1860. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8760-3_4

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  • DOI: https://doi.org/10.1007/978-1-4939-8760-3_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8759-7

  • Online ISBN: 978-1-4939-8760-3

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