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EPR Lineshape Analysis to Investigate the SNARE Folding Intermediates

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SNAREs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1860))

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Abstract

SNARE complex formation, which is believed to drive intracellular membrane fusion, transits through multiple conformational states along the membrane fusion pathway. The SNARE intermediates are biologically important because they serve as targets for fusion regulators and clostridial neurotoxins. Spin-labeling EPR has contributed significantly to the understanding of the structures and the dynamics of SNARE intermediates. In particular, the EPR lineshape analysis, which is highly sensitive to protein conformational changes such as the local coil-to-helix transition, has revealed the sequential compacting steps leading to formation of the highly stable four-helix bundle.

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Authors and Affiliations

  1. Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, USA

    Ryan Khounlo, Brenden J. D. Hawk & Yeon-Kyun Shin

Authors
  1. Ryan Khounlo
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  2. Brenden J. D. Hawk
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  3. Yeon-Kyun Shin
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Corresponding author

Correspondence to Yeon-Kyun Shin .

Editor information

Editors and Affiliations

  1. Department of Biochemistry, University of Illinois Urbana-Champaign, Urbana, IL, USA

    Rutilio Fratti

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Khounlo, R., Hawk, B.J.D., Shin, YK. (2019). EPR Lineshape Analysis to Investigate the SNARE Folding Intermediates. In: Fratti, R. (eds) SNAREs. Methods in Molecular Biology, vol 1860. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8760-3_3

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  • DOI: https://doi.org/10.1007/978-1-4939-8760-3_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8759-7

  • Online ISBN: 978-1-4939-8760-3

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