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Mass Spectrometry-Based Microbial Metabolomics: Techniques, Analysis, and Applications

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Microbial Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1859))

Abstract

The demand for understanding the roles genes play in biological systems has steered the biosciences into the direction the metabolome, as it closely reflects the metabolic activities within a cell. The importance of the metabolome is further highlighted by its ability to influence the genome, transcriptome, and proteome. Consequently, metabolomic information is being used to understand microbial metabolic networks. At the forefront of this work is mass spectrometry, the most popular metabolomics measurement technique. Mass spectrometry-based metabolomic analyses have made significant contributions to microbiological research in the environment and human disease. In this chapter, we break down the technical aspects of mass spectrometry-based metabolomics and discuss its application to microbiological research.

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Acknowledgments

The authors would also like to acknowledge that this work was part of the DOE Joint BioEnergy Institute (http://www.jbei.org) supported by the US Department of Energy, Office of Science, Office of Biological and Environmental Research, through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the US Department of Energy.

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  1. Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA

    Edward E. K. Baidoo & Veronica Teixeira Benites

  2. Joint BioEnergy Institute, Emeryville, California, USA

    Edward E. K. Baidoo & Veronica Teixeira Benites

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  1. Edward E. K. Baidoo
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  2. Veronica Teixeira Benites
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Correspondence to Edward E. K. Baidoo .

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  1. Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA, USA

    Edward E.K. Baidoo

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Baidoo, E.E.K., Teixeira Benites, V. (2019). Mass Spectrometry-Based Microbial Metabolomics: Techniques, Analysis, and Applications. In: Baidoo, E. (eds) Microbial Metabolomics. Methods in Molecular Biology, vol 1859. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8757-3_2

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  • DOI: https://doi.org/10.1007/978-1-4939-8757-3_2

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