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Microbial Metabolomics

Methods and Protocols

  • Edward E.K. Baidoo

Part of the Methods in Molecular Biology book series (MIMB, volume 1859)

Table of contents

  1. Front Matter
    Pages i-x
  2. Edward E. K. Baidoo
    Pages 1-8
  3. Microbial Metabolomics, Metabolism, and Physiology

    1. Front Matter
      Pages 9-9
    2. Edward E. K. Baidoo, Veronica Teixeira Benites
      Pages 11-69
  4. Metabolite Sample Preparation

  5. Current Analytical Techniques

    1. Front Matter
      Pages 153-153
    2. Christiane Gonçalves Campos, José Antônio de Aquino Ribeiro, João Ricardo Moreira de Almeida, Betania Ferraz Quirino, Patrícia Verardi Abdelnur
      Pages 155-169
    3. Edward E. K. Baidoo, George Wang, Chijioke J. Joshua, Veronica Teixeira Benites, Jay D. Keasling
      Pages 209-224
    4. David Rojo, Coral Barbas, Ángeles López-Gonzálvez
      Pages 253-260
  6. Data Analysis

  7. Back Matter
    Pages 347-351

About this book

Introduction

This detailed volume includes protocols that represent the breadth of microbial metabolomics approaches to both large-scale and small-scale experiments with intention of highlighting techniques that can be used for applications ranging from environmental microbiology to human disease. Utilizing mass spectrometry as their primary measurement tool, the chapters explore microbial metabolomics, metabolism and microbial physiology, metabolite sample preparation, current analytical techniques used to profile primary and secondary metabolites and lipids, as well as establishing data analysis workflows for targeted metabolomics, untargeted metabolomics, analysis of metabolic fluxes, and genome-scale models. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Microbial Metabolomics: Methods and Protocols serves as an ideal reference for both novice and advanced users and can be adapted to similar analytical platforms or customized to suit the needs of the researcher.

Keywords

Metabolite sample preparation Data analysis Metabolic networks Mass spectrometry Metabolome

Editors and affiliations

  • Edward E.K. Baidoo
    • 1
  1. 1.Joint BioEnergy InstituteLawrence Berkeley National LaboratoryEmeryvilleUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-8757-3
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8756-6
  • Online ISBN 978-1-4939-8757-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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