Abstract
The study of meningeal lymphatic vessels of the central nervous system (CNS) has recently gathered momentum, with several papers dissecting their role in draining solutes from cerebrospinal fluid and brain (Louveau et al., Nature 523(7560):337–341, 2015; Antila et al., J Exp Med 214(12):3645–3667, 2017; Aspelund et al., J Exp Med 212(7):991–999, 2015). Methodological capabilities, however, have been limited to few laboratories due to difficulties reproducibly visualizing these rare cell subsets in the meninges. To explore meningeal lymphatics fundamental role during homeostasis and how they may contribute to human pathology, the field has begun to require purification and characterization of lymphatic endothelial cells. Here, modern cell biological techniques involving a combination of histological, flow-cytometric, and functional drainage assays are applied to brain and spinal cord meninges and detailed stepwise procedures used for successful in vivo and ex vivo characterization of meningeal lymphatic vessels.
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References
Louveau A et al (2015) Structural and functional features of central nervous system lymphatic vessels. Nature 523(7560):337–341
Antila S et al (2017) Development and plasticity of meningeal lymphatic vessels. J Exp Med 214(12):3645–3667
Aspelund A et al (2015) A dural lymphatic vascular system that drains brain interstitial fluid and macromolecules. J Exp Med 212(7):991–999
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Herz, J., Louveau, A., Da Mesquita, S., Kipnis, J. (2018). Morphological and Functional Analysis of CNS-Associated Lymphatics. In: Oliver, G., Kahn, M. (eds) Lymphangiogenesis. Methods in Molecular Biology, vol 1846. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8712-2_9
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DOI: https://doi.org/10.1007/978-1-4939-8712-2_9
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Publisher Name: Humana Press, New York, NY
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