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Lymphangiogenesis

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Guillermo Oliver

    1. Center for Vascular & Developmental Biology, Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Mark L. Kahn

    1. Department of Medicine and Cardiovascular Institute, University of Pennsylvania, Philadelphia, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1846)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Three-Dimensional Visualization of the Lymphatic Vasculature

    • Cathrin Dierkes, Aaron Scherzinger, Friedemann Kiefer
    Pages 1-18

  3. Histological and Morphological Characterization of Developing Dermal Lymphatic Vessels

    • Kelly L. Betterman, Natasha L. Harvey
    Pages 19-35

  4. Genetic Lineage Tracing of Lymphatic Endothelial Cells in Mice

    • Ines Martinez-Corral, Taija Makinen
    Pages 37-53

  5. Visualization and Tools for Analysis of Zebrafish Lymphatic Development

    • Kazuhide S. Okuda, Sungmin Baek, Benjamin M. Hogan
    Pages 55-70

  6. Characterization of Zebrafish Facial Lymphatics

    • Tiffany C. Y. Eng, Jonathan W. Astin
    Pages 71-83

  7. Correlative Fluorescence and Scanning Electron Microscopy to Study Lymphovenous Valve Development

    • Xin Geng, R. Sathish Srinivasan
    Pages 85-96

  8. Characterization of Mouse Mesenteric Lymphatic Valve Structure and Function

    • Amélie Sabine, Michael J. Davis, Esther Bovay, Tatiana V. Petrova
    Pages 97-129

  9. Morphological Analysis of Lacteal Structure in the Small Intestine of Adult Mice

    • Sang Heon Suh, Seon Pyo Hong, Intae Park, Joo-Hye Song, Gou Young Koh
    Pages 131-139

  10. Morphological and Functional Analysis of CNS-Associated Lymphatics

    • Jasmin Herz, Antoine Louveau, Sandro Da Mesquita, Jonathan Kipnis
    Pages 141-151

  11. Morphological Analysis of Schlemm’s Canal in Mice

    • Benjamin R. Thomson, Susan E. Quaggin
    Pages 153-160

  12. Imaging Lymphatics in Mouse Lungs

    • Peter Baluk, Donald M. McDonald
    Pages 161-180

  13. Imaging of Endothelial Cell Dynamic Behavior in Zebrafish

    • Baptiste Coxam, Holger Gerhardt
    Pages 181-195

  14. Visualization and Measurement of Lymphatic Function In Vivo

    • Samia B. Bachmann, Michael Detmar, Steven T. Proulx
    Pages 197-211

  15. Investigating Effects of Fluid Shear Stress on Lymphatic Endothelial Cells

    • Daniel T. Sweet, Joshua D. Hall, John Welsh, Mark L. Kahn, Juan M. Jiménez
    Pages 213-227

  16. Methods for Assessing the Contractile Function of Mouse Lymphatic Vessels Ex Vivo

    • Jorge A. Castorena-Gonzalez, Joshua P. Scallan, Michael J. Davis
    Pages 229-248

  17. Evaluation and Characterization of Endothelial Cell Invasion and Sprouting Behavior

    • Jocelynda Salvador, George E. Davis
    Pages 249-259

  18. Dorsal Ear Skin Window for Intravital Imaging and Functional Analysis of Lymphangiogenesis

    • Witold W. Kilarski, Esra Güç, Melody A. Swartz
    Pages 261-277

  19. Isolation of Human Skin Lymphatic Endothelial Cells and 3D Reconstruction of the Lymphatic Vasculature In Vitro

    • Anita Rogic, Francois Auger, Mihaela Skobe
    Pages 279-290

  20. Stimulation and Inhibition of Lymphangiogenesis Via Adeno-Associated Viral Gene Delivery

    • Sinem Karaman, Harri Nurmi, Salli Antila, Kari Alitalo
    Pages 291-300
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Keywords

About this book

This volume discusses the latest tools, techniques, and animal models designed to study the processes of lymphatic vascular formation in vivo and in vitro and its functions in health and disease. The chapters in the book cover topics such as genetics lineage tracing of lymphatic endothelial cells in mice; characterization of zebrafish facial lymphatics; imaging lymphatics in mouse lungs; effects of fluid shear stress of lymphatic endothelial cells; and single cell mRNA sequencing of the mouse brain vasculature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Lymphangiogenesis: Methods and Protocols is a valuable resource to aid researchers with applying new approaches to answer their questions in this developing field.


Editors and Affiliations

  • Center for Vascular & Developmental Biology, Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, USA

    Guillermo Oliver

  • Department of Medicine and Cardiovascular Institute, University of Pennsylvania, Philadelphia, USA

    Mark L. Kahn

Bibliographic Information

  • Book Title: Lymphangiogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Guillermo Oliver, Mark L. Kahn

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8712-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8711-5Published: 22 September 2018

  • Softcover ISBN: 978-1-4939-9373-4Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8712-2Published: 21 September 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 345

  • Number of Illustrations: 12 b/w illustrations, 81 illustrations in colour

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

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