Abstract
Studies of lymphangiogenesis and lymphatic endothelial biology in vitro require pure cultures of lymphatic endothelial cells and 3D vascular constructs, which closely resemble native human lymphatic vasculature. We describe a method for the isolation of human dermal microvascular lymphatic endothelial cells and generation of a 3D lymphatic capillary network. The lymphatic vascular construct is generated by coculturing primary lymphatic endothelial cells and fibroblasts in their native matrix, without the use of synthetic scaffolds or exogenous factors. The tissue is stable over many weeks and accurately recapitulates features of human dermal lymphatic microvasculature.
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Acknowledgements
This work was supported by the DOD grant BC044819, the Susan G. Komen grant IBC17512321, by the DRPPP Pilot Project Program, Dept. Oncological Sciences, Mount Sinai (M.S.), and the CIHR grant SAC92850 and the Fondation d’entreprise SILAB-Jean Paufique (F.A.).
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Rogic, A., Auger, F., Skobe, M. (2018). Isolation of Human Skin Lymphatic Endothelial Cells and 3D Reconstruction of the Lymphatic Vasculature In Vitro. In: Oliver, G., Kahn, M. (eds) Lymphangiogenesis. Methods in Molecular Biology, vol 1846. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8712-2_18
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DOI: https://doi.org/10.1007/978-1-4939-8712-2_18
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