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Geometric Morphometrics as a Tool to Evaluate Teratogenic Effects in Zebrafish (Danio rerio)

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Teratogenicity Testing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1797))

Abstract

Geometric morphometrics allows for the characterization of shape using Cartesian geometric coordinates rather than linear or volumetric measurements, which are dependent upon size and are insufficient to capture geometric shape. By using landmarks on specimens, variations in position, orientation and scale between specimens can be removed to better compare variations in shape. This method has primarily been used in the fields of evolutionary biology and taxonomy. Here we describe how geometric morphometrics can be used to delineate variations in shape caused by teratogenic compounds in zebrafish.

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Acknowledgment

The authors thank Russell Minton for advice on choosing the easiest-to-use software for geometric morphometric analyses.

Author information

Authors and Affiliations

  1. Department of Biology, University of Texas of the Rio Grande Valley, Edinburg, TX, USA

    Kathryn E. Perez

  2. Department of Biology, University of Wisconsin – La Crosse, La Crosse, WI, USA

    Tisha C. King-Heiden

Authors
  1. Kathryn E. Perez
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  2. Tisha C. King-Heiden
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Corresponding author

Correspondence to Tisha C. King-Heiden .

Editor information

Editors and Affiliations

  1. Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal

    Luís Félix

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Perez, K.E., King-Heiden, T.C. (2018). Geometric Morphometrics as a Tool to Evaluate Teratogenic Effects in Zebrafish (Danio rerio). In: Félix, L. (eds) Teratogenicity Testing. Methods in Molecular Biology, vol 1797. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7883-0_20

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  • DOI: https://doi.org/10.1007/978-1-4939-7883-0_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7882-3

  • Online ISBN: 978-1-4939-7883-0

  • eBook Packages: Springer Protocols

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