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Cellular Responses in Drosophila melanogaster Following Teratogen Exposure

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Teratogenicity Testing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1797))

Abstract

Studies focusing on the teratogenicity of a series of new chemicals that are produced in a daily basis represent an important focus in toxicological/pharmaceutical research, particularly due to the risks arising from occupational exposure of the subjects. However, the complex mating procedures, scheduling of treatments, requirements for trained personnel, and elevated costs of traditional teratological assays with mammals hamper this type of assessments. Accordingly, the use of Drosophila melanogaster as a model for teratological studies has received considerable attention. Here some general protocols about Drosophila exposure—at different stages of their life cycle—to any chemical with putative teratological activity are presented. Importantly, some details about D. melanogaster embryonic, larval, pupal, or adult endpoints, that can be used to assess teratogenicity using flies as a model organism, are presented.

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Acknowledgments

The authors are grateful to FAPERGS, CAPES, CNPq, FINEP, INCT-EN, and UNIPAMPA. Additional support was given by CNPq/FAPERGS/DECIT/SCTIE-MS/PRONEM 16/2551-0000248-7 and CNPq (Universal) research grant # 449428/2014-1.

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Authors and Affiliations

  1. Universidade Federal do Pampa, Uruguaiana, RS, Brazil

    Matheus C. Bianchini, José L. R. Portela, Robson L. Puntel & Daiana S. Ávila

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  1. Matheus C. Bianchini
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  2. José L. R. Portela
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  3. Robson L. Puntel
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  4. Daiana S. Ávila
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Correspondence to Daiana S. Ávila .

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  1. Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal

    Luís Félix

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Bianchini, M.C., Portela, J.L.R., Puntel, R.L., Ávila, D.S. (2018). Cellular Responses in Drosophila melanogaster Following Teratogen Exposure. In: Félix, L. (eds) Teratogenicity Testing. Methods in Molecular Biology, vol 1797. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7883-0_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7883-0_13

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