Abstract
The synthetic photoaffinity probe designed to mimic bioactive molecules is one of the powerful tools for the identification of the target protein in living organisms. However, nonspecific interaction between the probe and nontargets would cause a misleading result in many cases of the photoaffinity labeling. In this chapter, we describe an enantiodifferential approach as a reliable method for the detection of the specific target protein of the bioactive natural product, jasmonate glucoside, a chemical factor that controls the nyctinastic leaf movement of the leguminous plants.
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Acknowledgements
We thank Dr. Y. Ishimaru (Tohoku Univ) for helpful discussion. This work was also supported in part by a Grant-in-Aid for Scientific Research (no. 23102012) on Innovative Areas “Chemical Biology of Natural Products” to M.U. from MEXT, Japan; a Grant-in-Aid for Scientific Research (nos. 26282207 and 17H00885 to M.U); JSPS A3 Foresight Program to M.U.; and JST (JPMJPR16Q4 to Y.T.).
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Takaoka, Y., Ueda, M. (2018). Enantiodifferential Approach for the Target Protein Detection of the Jasmonate Glucoside That Controls the Leaf Closure of Samanea saman. In: Fauser, F., Jonikas, M. (eds) Plant Chemical Genomics. Methods in Molecular Biology, vol 1795. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7874-8_13
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DOI: https://doi.org/10.1007/978-1-4939-7874-8_13
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