Abstract
Macrophages are one of the most abundant leucocytes in the intestinal mucosa where they are essential for maintaining homeostasis. However they are also implicated in the pathogenesis of disorders such as inflammatory bowel disease (IBD), offering potential targets for novel therapies.
Tissue macrophages are a heterogeneous population of immune cells that fulfill tissue-specific and niche-specific functions. These unique phenotypes likely reflect the heterogeneity of tissue macrophage origins and influence the tissue environment in which they reside. Here we describe how we can characterize and isolate the colonic macrophages.
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References
Bain CC, Mowat AM (2014) Macrophages in intestinal homeostasis and inflammation. Immunol Rev 260:102–117
Cader MZ, Kaser A (2013) Recent advances in inflammatory bowel disease: mucosal immune cells in intestinal inflammation. Gut 62:1653–1664
Cerovic V, Bain CC, Mowat AM et al (2014) Intestinal macrophages and dendritic cells: what's the difference? Trends Immunol 35:270–277
Hashimoto D, Chow A, Noizat C et al (2013) Tissue-resident macrophages self-maintain locally throughout adult life with minimal contribution from circulating monocytes. Immunity 38:792–804
Kierdorf K, Prinz M, Geissmann F et al (2015) Development and function of tissue resident macrophages in mice. Semin Immunol 27:369–378
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Petit, V. (2018). Isolation and Phenotyping of Intestinal Macrophages. In: Rousselet, G. (eds) Macrophages. Methods in Molecular Biology, vol 1784. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7837-3_9
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DOI: https://doi.org/10.1007/978-1-4939-7837-3_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7836-6
Online ISBN: 978-1-4939-7837-3
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