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Model Phospholipid Liposomes to Study the β-Amyloid-Peptide-Induced Membrane Disruption

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Peptide Self-Assembly

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1777))

Abstract

Model phospholipid liposomes have been utilized widely to study the molecular interactions between peptides and membrane bilayers. In the mechanistic study of Alzheimer’s disease (AD), disruption of neuronal cell membranes has been considered as a major contribution for the β-amyloid (Aβ) peptides’ neurotoxicity. However, clear interpretation of the Aβ-induced cellular membrane at high-resolution level is challenging because of the co-existence of multiple pathways. Here we present the generation of simplified model liposome systems that will facilitate the in-depth mechanistic studies. Protocols for the preparation of model liposomes and the characterization of individual membrane disruption effects will be described.

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Acknowledgments

This work is supported by the start-up funding from the Research Foundation of the State University of New York. We acknowledge Dr. He Dong and Mr. Dawei Xu from Clarkson University, and Dr. Christof Grewer from Binghamton University for their kind helps on fluorescence imaging and electrophysiological current measurements.

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Authors and Affiliations

  1. Department of Chemistry, State University of New York at Binghamton, Binghamton, NY, USA

    Wei Qiang & Katelynne E. Doherty

Authors
  1. Wei Qiang
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  2. Katelynne E. Doherty
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Corresponding author

Correspondence to Wei Qiang .

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Editors and Affiliations

  1. Department of Chemistry, University of Rochester, Rochester, New York, USA

    Bradley L. Nilsson

  2. Department of Medicinal Chemistry, Institute for Translational Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA

    Todd M. Doran

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Qiang, W., Doherty, K.E. (2018). Model Phospholipid Liposomes to Study the β-Amyloid-Peptide-Induced Membrane Disruption. In: Nilsson, B., Doran, T. (eds) Peptide Self-Assembly. Methods in Molecular Biology, vol 1777. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7811-3_23

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  • DOI: https://doi.org/10.1007/978-1-4939-7811-3_23

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7809-0

  • Online ISBN: 978-1-4939-7811-3

  • eBook Packages: Springer Protocols

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