Model Phospholipid Liposomes to Study the β-Amyloid-Peptide-Induced Membrane Disruption
Model phospholipid liposomes have been utilized widely to study the molecular interactions between peptides and membrane bilayers. In the mechanistic study of Alzheimer’s disease (AD), disruption of neuronal cell membranes has been considered as a major contribution for the β-amyloid (Aβ) peptides’ neurotoxicity. However, clear interpretation of the Aβ-induced cellular membrane at high-resolution level is challenging because of the co-existence of multiple pathways. Here we present the generation of simplified model liposome systems that will facilitate the in-depth mechanistic studies. Protocols for the preparation of model liposomes and the characterization of individual membrane disruption effects will be described.
Key wordsβ-Amyloid peptides Phospholipid liposomes Membrane disruption Fibrillation lipid mixing Electrophysiology Ion channels
This work is supported by the start-up funding from the Research Foundation of the State University of New York. We acknowledge Dr. He Dong and Mr. Dawei Xu from Clarkson University, and Dr. Christof Grewer from Binghamton University for their kind helps on fluorescence imaging and electrophysiological current measurements.
- 14.Qiang W, Akinlolu RD, Nam M, Shu N (2014) Strcutral evolution and membrane interaction of the 40-residue beta amyloid peptides: differences in the initial proximity between peptides and the membrane bilayer studied by solid-state nulear magnetic resonance spectroscopy. Biochemistry 53(48):7503–7514CrossRefGoogle Scholar