Model Phospholipid Liposomes to Study the β-Amyloid-Peptide-Induced Membrane Disruption

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1777)

Abstract

Model phospholipid liposomes have been utilized widely to study the molecular interactions between peptides and membrane bilayers. In the mechanistic study of Alzheimer’s disease (AD), disruption of neuronal cell membranes has been considered as a major contribution for the β-amyloid (Aβ) peptides’ neurotoxicity. However, clear interpretation of the Aβ-induced cellular membrane at high-resolution level is challenging because of the co-existence of multiple pathways. Here we present the generation of simplified model liposome systems that will facilitate the in-depth mechanistic studies. Protocols for the preparation of model liposomes and the characterization of individual membrane disruption effects will be described.

Key words

β-Amyloid peptides Phospholipid liposomes Membrane disruption Fibrillation lipid mixing Electrophysiology Ion channels 

Notes

Acknowledgments

This work is supported by the start-up funding from the Research Foundation of the State University of New York. We acknowledge Dr. He Dong and Mr. Dawei Xu from Clarkson University, and Dr. Christof Grewer from Binghamton University for their kind helps on fluorescence imaging and electrophysiological current measurements.

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of ChemistryState University of New York at BinghamtonBinghamtonUSA

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