Skip to main content
SpringerLink
Log in

Microwave-Assisted Synthesis and Immunological Evaluation of Self-Assembling Peptide Vaccines

  • Protocol
  • First Online:
Peptide Self-Assembly

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1777))

Abstract

Self-assembling peptides spontaneously associate into functional supramolecular scaffolds, which have found numerous biomedical applications. These molecular assemblies have applications in nerve regeneration, wound healing, and both prophylactic and therapeutic vaccination. They can also be useful tools for proliferation assays, sustained culture of difficult cell lines, or activation of cell lines for immunoassays. This protocol will describe the basic peptide synthesis and purification of model self-assembling peptide immunogen and methods for vaccinating mice, collecting lymph nodes, and stimulating cells ex vivo.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Mora-Solano C, Collier JH (2014) Engaging adaptive immunity with biomaterials. J Mater Chem B Mater Biol Med 2(17):2409–2421

    Article  CAS  PubMed  Google Scholar 

  2. Khan TA, Reddy ST (2014) Immunological principles regulating immunomodulation with biomaterials. Acta Biomater 10(4):1720–1727

    Article  CAS  PubMed  Google Scholar 

  3. Petkau-Milroy K, Brunsveld L (2013) Supramolecular chemical biology; bioactive synthetic self-assemblies. Org Biomol Chem 11(2):219–232

    Article  CAS  PubMed  Google Scholar 

  4. Bowerman CJ, Nilsson BL (2012) Self-assembly of amphipathic beta-sheet peptides: insights and applications. Biopolymers 98(3):169–184

    Article  CAS  PubMed  Google Scholar 

  5. Mart RJ, Osborne RD, Stevens MM, Ulijn RV (2006) Peptide-based stimuli-responsive biomaterials. Soft Matter 2(10):822–835

    Article  CAS  PubMed  Google Scholar 

  6. Jung JP, Gasiorowski JZ, Collier JH (2010) Fibrillar peptide gels in biotechnology and biomedicine. Biopolymers 94(1):49–59

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Rudra JS, Tian YF, Jung JP, Collier JH (2010) A self-assembling peptide acting as an immune adjuvant. Proc Natl Acad Sci U S A 107(2):622–627

    Article  CAS  PubMed  Google Scholar 

  8. Rudra JS, Tripathi PK, Hildeman DA, Jung JP, Collier JH (2010) Immune responses to coiled coil supramolecular biomaterials. Biomaterials 31(32):8475–8483

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Hudalla GA, Modica JA, Tian YF, Rudra JS, Chong AS, Sun T, Mrksich M, Collier JH (2013) A self-adjuvanting supramolecular vaccine carrying a folded protein antigen. Adv Healthc Mater 2(8):1114–1119

    Article  CAS  PubMed  Google Scholar 

  10. Pompano RR, Chen JJ, Verbus EA, Han HF, Fridman A, McNeely T, Collier JH, Chong AS (2014) Titrating T-cell epitopes within self-assembled vaccines optimizes CD4+helper T cell and antibody outputs. Adv Healthc Mater 3(11):1898–1908

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Li XD, Galliher-Beckley A, Huang HZ, Sun XZ, Shi JS (2013) Peptide nanofiber hydrogel adjuvanted live virus vaccine enhances cross-protective immunity to porcine reproductive and respiratory syndrome virus. Vaccine 31(41):4508–4515

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Wang HM, Luo Z, Wang YCZ, He T, Yang CB, Ren CH, Ma LS, Gong CY, Li XY, Yang ZM (2016) Enzyme-catalyzed formation of supramolecular hydrogels as promising vaccine adjuvants. Adv Funct Mater 26(11):1822–1829

    Article  CAS  Google Scholar 

  13. Grenfell RFQ, Shollenberger LM, Samli EF, Harn DA (2015) Vaccine self-assembling immune matrix is a new delivery platform that enhances immune responses to recombinant HBsAg in mice. Clin Vaccine Immunol 22(3):336–343

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Snook JD, Chesson CB, Peniche AG, Dann SM, Paulucci A, Pinchuk IV, Rudra JS (2016) Peptide nanofiber-CaCO3 composite microparticles as adjuvant-free oral vaccine delivery vehicles. J Mater Chem B 4(9):1640–1649

    Article  CAS  PubMed  Google Scholar 

  15. Rudra JS, Mishra S, Chong AS, Mitchell RA, Nardin EH, Nussenzweig V, Collier JH (2012) Self-assembled peptide nanofibers raising durable antibody responses against a malaria epitope. Biomaterials 33(27):6476–6484

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Huang ZH, Shi L, Ma JW, Sun ZY, Cai H, Chen YX, Zhao YF, Li YM (2012) A totally synthetic, self-assembling, adjuvant-free MUC1 glycopeptide vaccine for cancer therapy. J Am Chem Soc 134(21):8730–8733

    Article  CAS  PubMed  Google Scholar 

  17. Chesson CB, Huelsmann EJ, Lacek AT, Kohlhapp FJ, Webb MF, Nabatiyan A, Zloza A, Rudra JS (2014) Antigenic peptide nanofibers elicit adjuvant-free CD8(+) T cell responses. Vaccine 32(10):1174–1180

    Article  CAS  PubMed  Google Scholar 

  18. Rudra JS, Ding Y, Neelakantan H, Ding C, Appavu R, Stutz S, Snook JD, Chen H, Cunningham KA, Zhou J (2016) Suppression of cocaine-evoked hyperactivity by self-adjuvanting and multivalent peptide nanofiber vaccines. ACS Chem Neurosci 7(5):546–552

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacology & Toxicology, University of Texas Medical Branch, Galveston, TX, USA

    C. Brent Chesson, Rojelio Elias Alvarado & Jai S. Rudra

  2. Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX, USA

    C. Brent Chesson & Jai S. Rudra

Authors
  1. C. Brent Chesson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rojelio Elias Alvarado
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jai S. Rudra
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jai S. Rudra .

Editor information

Editors and Affiliations

  1. Department of Chemistry, University of Rochester, Rochester, New York, USA

    Bradley L. Nilsson

  2. Department of Medicinal Chemistry, Institute for Translational Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA

    Todd M. Doran

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Brent Chesson, C., Alvarado, R.E., Rudra, J.S. (2018). Microwave-Assisted Synthesis and Immunological Evaluation of Self-Assembling Peptide Vaccines. In: Nilsson, B., Doran, T. (eds) Peptide Self-Assembly. Methods in Molecular Biology, vol 1777. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7811-3_15

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-7811-3_15

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7809-0

  • Online ISBN: 978-1-4939-7811-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation