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Nanomanufacture of Free-Standing, Porous, Janus-Type Films of Polymer–Plant Virus Nanoparticle Arrays

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Virus-Derived Nanoparticles for Advanced Technologies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1776))

Abstract

We present a facile method for preparing hierarchical assemblies of cowpea mosaic virus (CPMV) nanoparticles adsorbed onto patterned polypyrrole copolymer arrays, which can be released as a freely standing and microporous polymer–protein membrane with a Janus-type structure. The patterning protocol is based on colloidal sphere lithography wherein a sacrificial honeycomb pattern composed of colloidal polystyrene (PS) microspheres is assembled on an electrode. A thin layer of polypyrrole film is electropolymerized within the interstices of the template and monitored using an electrochemical quartz crystal microbalance with dissipation (EC-QCM-D) and microscopy. Dissolving the PS template reveals an inverse opaline pattern capable of electrostatically capturing the CPMV particles. Through an electrochemical trigger, the polypyrrole–CPMV delaminates from the surface producing a self-sustaining polymer–protein membrane that can potentially be used for sensing and nanocargo applications.

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Acknowledgments

This work was supported in part by the National Science Foundation (CMMI NM 1333651 to NFS and RCA and STC-0423914 to RCA). Case Farm is acknowledged for scaled-up plant production. The authors also acknowledge technical support from Biolin Scientific Inc. and the Materials for Opto/Electronics Research and Education (MORE) Center at the Case Western Reserve University.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USA

    Brylee David B. Tiu, Rigoberto C. Advincula & Nicole F. Steinmetz

  2. Department of Macromolecular Science and Engineering, Case Western Reserve University, Cleveland, OH, USA

    Brylee David B. Tiu, Rigoberto C. Advincula & Nicole F. Steinmetz

  3. Department of Materials Science and Engineering, Case Western Reserve University, Cleveland, OH, USA

    Nicole F. Steinmetz

  4. Department of Radiology, Case Western Reserve University, Cleveland, OH, USA

    Nicole F. Steinmetz

  5. Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH, USA

    Nicole F. Steinmetz

Authors
  1. Brylee David B. Tiu
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  2. Rigoberto C. Advincula
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  3. Nicole F. Steinmetz
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Corresponding authors

Correspondence to Rigoberto C. Advincula or Nicole F. Steinmetz .

Editor information

Editors and Affiliations

  1. Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Stuttgart, Germany

    Christina Wege

  2. Department of Biological Chemistry, John Innes Centre, Norwich, United Kingdom

    George P. Lomonossoff

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Tiu, B.D.B., Advincula, R.C., Steinmetz, N.F. (2018). Nanomanufacture of Free-Standing, Porous, Janus-Type Films of Polymer–Plant Virus Nanoparticle Arrays. In: Wege, C., Lomonossoff, G. (eds) Virus-Derived Nanoparticles for Advanced Technologies. Methods in Molecular Biology, vol 1776. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7808-3_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7808-3_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7806-9

  • Online ISBN: 978-1-4939-7808-3

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