Abstract
Plant viral nanoparticles (VNPs) are currently being developed as novel vessels for delivery of diagnostic and therapeutic cargos to sites of disease. With a rapid increase in the number of VNP variants and their potential applications in nanomedicine, the properties they acquire in the bloodstream need to be investigated. Biomolecules present in plasma are known to adsorb onto the surface of nanomaterials (including VNPs), forming a biointerface called the protein corona, which is capable of reprogramming the properties of VNPs. Here we describe a few general methods to isolate and study the VNP–protein corona complexes, in order to evaluate the impact of protein corona on molecular recognition of VNPs by target cells, and clearance by phagocytes. We outline procedures for in vivo screening of VNP fates in a mouse model, which may be useful for evaluation of efficacy and biocompatibility of different VNP based formulations.
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Acknowledgment
This work was supported in part by a grant from the National Science Foundation CAREER DMR 1452257 (to NFS) and grants from the National Institute of Health (NIH): NHLBI R21 HL121130 (to NFS) and a pilot grant from Case-Coulter Translational Research Partnership and the Harrington Heart & Vascular Institute.
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Pitek, A.S., Veliz, F.A., Jameson, S.A., Steinmetz, N.F. (2018). Interactions Between Plant Viral Nanoparticles (VNPs) and Blood Plasma Proteins, and Their Impact on the VNP In Vivo Fates. In: Wege, C., Lomonossoff, G. (eds) Virus-Derived Nanoparticles for Advanced Technologies. Methods in Molecular Biology, vol 1776. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7808-3_38
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DOI: https://doi.org/10.1007/978-1-4939-7808-3_38
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