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Drug-Loaded Plant-Virus Based Nanoparticles for Cancer Drug Delivery

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Virus-Derived Nanoparticles for Advanced Technologies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1776))

Abstract

Nature has designed nanosized particles, specifically viruses, equipped to deliver cargo to cells. We report the chemical bioconjugation and shape shifting of a hollow, rod-shaped tobacco mosaic virus (TMV) to dense spherical nanoparticles (SNPs). We describe methods to transform TMV rods to spheres, load TMV rods and spheres with the chemotherapeutic drug, doxorubicin (DOX), to deliver modified particles to breast cancer cells, and to determine the IC50 values of the plant virus-based drug delivery system.

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Acknowledgments

This work was supported in part by a grant from the National Science Foundation (DMR 1452257 to N.F.S.).

Author information

Authors and Affiliations

  1. Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USA

    Michael A. Bruckman & Nicole F. Steinmetz

  2. NanoBio Systems, Elyria, OH, USA

    Michael A. Bruckman

  3. Department of Pathology, Case Western Reserve University, Cleveland, OH, USA

    Anna E. Czapar

  4. Department of Radiology, Case Western Reserve University, Cleveland, OH, USA

    Nicole F. Steinmetz

  5. Department of Materials Science and Engineering, Case Western Reserve University, Cleveland, OH, USA

    Nicole F. Steinmetz

  6. Department of Macromolecular Science and Engineering, Case Western Reserve University, Cleveland, OH, USA

    Nicole F. Steinmetz

  7. Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH, USA

    Nicole F. Steinmetz

Authors
  1. Michael A. Bruckman
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  2. Anna E. Czapar
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  3. Nicole F. Steinmetz
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Corresponding author

Correspondence to Nicole F. Steinmetz .

Editor information

Editors and Affiliations

  1. Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Stuttgart, Germany

    Christina Wege

  2. Department of Biological Chemistry, John Innes Centre, Norwich, United Kingdom

    George P. Lomonossoff

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Bruckman, M.A., Czapar, A.E., Steinmetz, N.F. (2018). Drug-Loaded Plant-Virus Based Nanoparticles for Cancer Drug Delivery. In: Wege, C., Lomonossoff, G. (eds) Virus-Derived Nanoparticles for Advanced Technologies. Methods in Molecular Biology, vol 1776. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7808-3_28

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  • DOI: https://doi.org/10.1007/978-1-4939-7808-3_28

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7806-9

  • Online ISBN: 978-1-4939-7808-3

  • eBook Packages: Springer Protocols

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