Abstract
Empty (containing no genomic material) CPMV virus-like particles are loaded within the virus capsid with metal or metal oxide. Metal ions are allowed to diffuse through pores in the capsid surface and are reduced or hydrolyzed to metallic nanoparticles. The external surface of the virus-like particles remains amenable to further chemical modification.
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References
Lin T, Chen Z, Usha R et al (1999) The refined crystal structure of cowpea mosaic virus at 2.8 Å resolution. Virology 265(1):20–34
Lin T, Johnson JE (2003) Structures of picorna-like plant viruses: implications and applications. Adv Virus Res 62:167–239
Lomonossoff GP, Johnson JE (1991) The synthesis and structure of comovirus capsids. Prog Biophys Mol Biol 55(2):107–137
Saunders K, Sainsbury F, Lomonossoff GP (2009) Efficient generation of cowpea mosaic virus empty virus-like particles by the proteolytic processing of precursors in insect cells and plants. Virology 393(2):329–337
Sainsbury F, Thuenemann EC, Lomonossoff GP (2009) pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants. Plant Biotechnol J 7(7):682–693
Sainsbury F, Canizares MC, Lomonossoff GP (2010) Cowpea mosaic virus: the plant virus-based biotechnology workhorse. Annu Rev Phytopathol 48:437–455
Sainsbury F, Saxena P, Aljabali AA, Saunders K, Evans DJ, Lomonossoff GP (2014) Genetic engineering and characterization of cowpea mosaic virus empty virus-like particles. Methods Mol Biol 1108:139–153
Sainsbury F, Lomonossoff GP (2008) Extremely high-level and rapid transient protein production in plants without the use of viral replication. Plant Physiol 148(3):1212–1218
Hesketh EL, Meshcheriakova Y, Dent KC, Saxena P, Thompson RF, Cockburn JJ, Lomonossoff GP, Ranson NA (2015) Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM. Nat Commun 6:10113–10119
Wen AM, Shukla S, Saxena P et al (2012) Interior engineering of a viral nanoparticle and its tumor homing properties. Biomacromolecules 13(12):3990–4001
Aljabali AAA, Sainsbury F, Lomonossoff G et al (2010) Cowpea mosaic virus unmodified empty viruslike particles loaded with metal and metal oxide. Small 6(7):818–821
Sainsbury F, Saunders K, Aljabali A et al (2011) Peptide-controlled access to the interior surface of empty virus nanoparticles. ChemBioChem 12(16):2435–2440
Jaafar M, Aljabali AA, Berlanga I et al (2014) Structural insights into magnetic clusters grown inside virus capsids. ACS Appl Mater Interfaces 6(23):20936–20942
Wellink J (1998) Comovirus isolation and RNA extraction. Methods Mol Biol 81:205–209
Steinmetz N, Calder G, Lomonossoff G et al (2006) Plant viral capsids as nanobuilding blocks: construction of arrays on solid supports. Langmuir 22(24):10032–10037
van Kammen A (1967) Purification and properties of the components of cowpea mosaic virus. Virology 31(4):633–642
Acknowledgment
This work was supported by the Biotechnology and Biological Sciences Research Council, UK (Core Strategic Grant to the John Innes Centre, D.J.E., and JIC DTG, A.A.A.A.).
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Aljabali, A.A.A., Evans, D.J. (2018). Internal Deposition of Cobalt Metal and Iron Oxide Within CPMV eVLPs. In: Wege, C., Lomonossoff, G. (eds) Virus-Derived Nanoparticles for Advanced Technologies. Methods in Molecular Biology, vol 1776. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7808-3_12
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DOI: https://doi.org/10.1007/978-1-4939-7808-3_12
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