Abstract
Adipose-derived stem cells are capable of self-renewal and differentiation along multiple cell lineages, and have potential applications in a wide range of therapies. ASCs are commonly cultured as monolayers on tissue culture plastic, but there are indications that they may lose their cell-specific properties with time in vitro. There has been a growing interest in culturing adherent cells using three-dimensional techniques based on the understanding that growing cells on plastic surfaces cannot truly recapitulate 3D in vivo conditions. Here we describe a novel method for generating and culturing rabbit ASCs as scaffold-free 3D cell aggregates using micropatterned wells via a forced aggregation technique.
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Acknowledgments
This work was supported by the US Army Medical Research and Material Command (W81XWH-10-2-0054).
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The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or reflecting the views of the Department of Defense or US Government. One of the authors (KPL) is an employee of the US Government, and this work was prepared as part of his official duties.
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Rettinger, C.L., Leung, K.P., Chan, R.K. (2018). Scaffold-Free, Size-Controlled Three-Dimensional Culture of Rabbit Adipose-Derived Stem Cells. In: Bunnell, B.A., Gimble, J.M. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 1773. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7799-4_3
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DOI: https://doi.org/10.1007/978-1-4939-7799-4_3
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