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Evaluation of the Purity of Sertoli Cell Primary Cultures

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Sertoli Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1748))

Abstract

The purity of the primary cultures of Sertoli cells is a factor for the validation of the studies using this methodological approach. There is a probability of contamination of these cultures with other testicular cellular types, such as peritubular cells and germ cells (that represent a large percentage of the volume of the seminiferous tubules).

For the evaluation of the purity of cultures, the immunocytochemistry technique (immunoperoxidase or immunofluorescence) is frequently used to label a protein marker specific for the Sertoli cells within the testicular environment. The expression of several protein markers can be used, with different particularities, being that vimentin is often used as a marker for Sertoli cells. Vimentin is expressed independently of the differentiation state of the cells and is observed around the nuclei and in the cytoplasm of Sertoli cells.

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Acknowledgments

This work was supported by the “Fundação para a Ciência e a Tecnologia” (FCT) (PTDC/BBB-BQB/1368/2014) and UMIB (PEst-OE/SAU/UI0215/2014). R.L. Bernardino was also financed by FCT (SFRH/BD/103105/2014).

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Authors and Affiliations

  1. Unit for Multidisciplinary Research in Biomedicine (UMIB), Laboratory of Cell Biology, Department of Microscopy, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal

    Raquel L. Bernardino & Marco G. Alves

  2. Department of Microscopy, Laboratory of Cell Biology and Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal

    Pedro F. Oliveira

  3. Department of Genetics, Faculty of Medicine, University of Porto, Porto, Portugal

    Pedro F. Oliveira

  4. i3S - Instituto de Investigação e Inovação em Saúde, University of Porto, Porto, Portugal

    Pedro F. Oliveira

  5. Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari “Aldo Moro”, Bari, Italy

    Pedro F. Oliveira

Authors
  1. Raquel L. Bernardino
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  2. Marco G. Alves
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  3. Pedro F. Oliveira
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Corresponding author

Correspondence to Pedro F. Oliveira .

Editor information

Editors and Affiliations

  1. Department of Microscopy, Laboratory of Cell Biology and Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal

    Marco G. Alves

  2. Department of Microscopy, Laboratory of Cell Biology and Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal

    Pedro F. Oliveira

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Bernardino, R.L., Alves, M.G., Oliveira, P.F. (2018). Evaluation of the Purity of Sertoli Cell Primary Cultures. In: Alves, M., Oliveira, P. (eds) Sertoli Cells. Methods in Molecular Biology, vol 1748. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7698-0_2

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  • DOI: https://doi.org/10.1007/978-1-4939-7698-0_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7697-3

  • Online ISBN: 978-1-4939-7698-0

  • eBook Packages: Springer Protocols

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