Sertoli Cells

Methods and Protocols

  • Marco G. Alves
  • Pedro F. Oliveira

Part of the Methods in Molecular Biology book series (MIMB, volume 1748)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Raquel L. Bernardino, Marco G. Alves, Pedro F. Oliveira
    Pages 1-8
  3. Raquel L. Bernardino, Marco G. Alves, Pedro F. Oliveira
    Pages 9-15
  4. Barbara Bilinska, Anna Hejmej, Malgorzata Kotula-Balak
    Pages 17-36
  5. Jesús Martínez-Hernández, Vicente Seco-Rovira, Ester Beltrán-Frutos, Victor Quesada-Cubo, Concepción Ferrer, Luis Miguel Pastor
    Pages 49-60
  6. Maria João Freitas, Margarida Fardilha
    Pages 61-71
  7. Maria João Freitas, Margarida Fardilha
    Pages 73-84
  8. Marina G. Yefimova, Nadia Messaddeq, Annie-Claire Meunier, Anne Cantereau, Bernard Jegou, Nicolas Bourmeyster
    Pages 85-101
  9. Nabil Eid, Yuko Ito, Akio Horibe, Hitomi Hamaoka, Yoichi Kondo
    Pages 103-112
  10. Chao Liu, Jehangir Khan, Wei Li
    Pages 113-128
  11. Luís Crisóstomo, Marco G. Alves, Agostina Gorga, Mário Sousa, María F. Riera, María N. Galardo et al.
    Pages 129-155
  12. Ivana Jarak, Pedro F. Oliveira, Gustavo Rindone, Rui A. Carvalho, María N. Galardo, María F. Riera et al.
    Pages 157-171
  13. Rita Ferreira, Fábio Trindade, Rui Vitorino
    Pages 173-190
  14. Hong Wang, Qingqing Yuan, Minghui Niu, Liping Wen, Hongyong Fu, Fan Zhou et al.
    Pages 191-202
  15. Diane Rebourcet, Annalucia Darbey, Michael Curley, Peter O’Shaughnessy, Lee B. Smith
    Pages 203-228
  16. Qing Wen, Elizabeth I. Tang, Nan Li, Dolores D. Mruk, Will M. Lee, Bruno Silvestrini et al.
    Pages 229-243
  17. Haiqi Chen, Wing-yee Lui, Dolores D. Mruk, Xiang Xiao, Renshan Ge, Qingquan Lian et al.
    Pages 245-252
  18. Pranitha Jenardhanan, Manivel Panneerselvam, Premendu P. Mathur
    Pages 253-277
  19. Anna Maggio, Raquel L. Bernardino, Patrizia Gena, Marco G. Alves, Pedro F. Oliveira, Giuseppe Calamita
    Pages 279-286

About this book

Introduction

This detailed volume explores the use of Sertoli cells as models in male reproductive biology or as supporters for other cell types, and it answers the need for standardized protocols for their in vitro culture. Prepared for non-specialists as well as experienced researchers that may be interested in a multidisciplinary approach to study these cells, the book examines these remarkable cells that present some unique features that go far beyond the physical and nutritional support of spermatogenesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Sertoli Cells: Methods and Protocols serves as a valuable resource for experienced researchers in reproductive biology, particularly andrologists, but also for future investigators and young students starting to study these extraordinary cells.

Keywords

Andrology Cell culture Spermatogenesis Reproductive biology Somatic testicular cells

Editors and affiliations

  • Marco G. Alves
    • 1
  • Pedro F. Oliveira
    • 2
  1. 1.Department of Microscopy, Laboratory of Cell Biology and Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS)University of PortoPortoPortugal
  2. 2.Department of Microscopy, Laboratory of Cell Biology and Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS)University of PortoPortoPortugal

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7698-0
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7697-3
  • Online ISBN 978-1-4939-7698-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
Industry Sectors
Biotechnology
Consumer Packaged Goods
Pharma