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Pulse-Chase Biosynthetic Radiolabeling of Pancreatic Islets to Measure Beta Cell Function

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Investigations of Early Nutrition Effects on Long-Term Health

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1735))

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Abstract

Pulse-chase radiolabeling of cells with radioactive amino acids is a common method for studying the biosynthesis of proteins. The labeled proteins can then be immunoprecipitated and analyzed by electrophoresis and gel imaging techniques. This chapter presents a protocol for the biosynthetic labeling and immunoprecipitation of pancreatic islet proteins which are known to be affected in disorders such as diabetes, obesity, and metabolic syndrome.

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Authors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil

    Paul C. Guest

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  1. Paul C. Guest
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Correspondence to Paul C. Guest .

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Editors and Affiliations

  1. Debden, Essex, United Kingdom

    Paul C. Guest

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Guest, P.C. (2018). Pulse-Chase Biosynthetic Radiolabeling of Pancreatic Islets to Measure Beta Cell Function. In: Guest, P. (eds) Investigations of Early Nutrition Effects on Long-Term Health. Methods in Molecular Biology, vol 1735. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7614-0_22

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  • DOI: https://doi.org/10.1007/978-1-4939-7614-0_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7613-3

  • Online ISBN: 978-1-4939-7614-0

  • eBook Packages: Springer Protocols

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