Abstract
Mental disorders are generally characterized by a combination of abnormal thoughts, perceptions, emotions, behavior, and relationships with others. Although multiple risk factors, such as genetic and environmental factors and interaction of these factors, are suggested, the exact etiologies are not known. On the other hand, it has been strongly suggested that the dopaminergic system is impaired in a variety of mental disorders. In the described method, the SH-SY5Y neuroblastoma cell line is differentiated to neuronal cell which expresses NSE, neuronal marker, and dopamine transporter (DAT) by treatment with all-trans-retinoic acid. SH-SY5Y cells allow investigating neuronal phenotypes of mental disorders as an in vitro model of these disorders.
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Acknowledgments
This work was supported in part by the Japan Society for the Promotion of Science (JSPS) Program for Advancing Strategic International Networks to Accelerate the Circulation of Talented Researchers, Grant No. S2603, and the Japan Foundation for Pediatric Research (to K.I.).
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Iwata, K. (2018). Studies of a Neuronal Cell Line as a Model of Psychiatric Disorders. In: Guest, P. (eds) Investigations of Early Nutrition Effects on Long-Term Health. Methods in Molecular Biology, vol 1735. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7614-0_13
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DOI: https://doi.org/10.1007/978-1-4939-7614-0_13
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