Abstract
The avian chorioallantoic membrane (CAM) is a simple, highly vascularized extraembryonic membrane, which performs multiple functions during embryonic development. Therefore, the models of chicken and quail assays represent robust experimental platforms to study angiogenesis, which reflects perturbation of the entire vascular tree. This experimental approach, when combined with fractal morphometry, is sensitive to changes in vascular branching pattern and density. Nerve growth factor is a neurotrophin promoting angiogenesis in CAM models. Here, we provide a detailed protocol of the quail CAM, shell-less model, to study nerve growth factor effects on blood capillary sprouting. The quail CAM assay may be beneficial in investigations of cellular and molecular aspects of neurotrophin-induced angiogenesis and for developing novel anti-angiogenesis and anticancer therapies.
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Acknowledgments
Philip Lazarovici holds the Jacob Gitlin Chair in Physiology and is affiliated with and supported by the David R. Bloom Center for Pharmacy and the Adolph and Klara Brettler Medical Research Center at the Hebrew University of Jerusalem, Israel. Peter I. Lelkes is the Laura H. Carnell Professor of Bioengineering and Chair, Dept. Bioengineering, Temple University. Research reported in this publication was supported in part by a grant from the Temple University Moulder Center for Drug Discovery (CM and PIL) and a Research Bridge Funding Award (PIL) from the Temple University Office of the Vice President for Research Administration (OVPR).
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Lazarovici, P., Lahiani, A., Gincberg, G., Haham, D., Marcinkiewicz, C., Lelkes, P.I. (2018). Nerve Growth Factor-Induced Angiogenesis: 2. The Quail Chorioallantoic Membrane Assay. In: Skaper, S. (eds) Neurotrophic Factors. Methods in Molecular Biology, vol 1727. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7571-6_19
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DOI: https://doi.org/10.1007/978-1-4939-7571-6_19
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