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Neurotrophic Factors

Methods and Protocols

  • Stephen D. Skaper

Part of the Methods in Molecular Biology book series (MIMB, volume 1727)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Stephen D. Skaper
    Pages 1-17
  3. Laura Facci, Stephen D. Skaper
    Pages 39-47
  4. Stephen D. Skaper, Massimo Barbierato, Vanni Ferrari, Morena Zusso, Laura Facci
    Pages 107-118
  5. Laura Facci, Massimo Barbierato, Stephen D. Skaper
    Pages 127-137
  6. Cinzia Volonté, Adele De Ninno, Susanna Amadio
    Pages 139-154
  7. Massimo Barbierato, Stephen D. Skaper, Laura Facci
    Pages 155-166
  8. M. Foster Olive, Armani P. Del Franco, Cassandra D. Gipson
    Pages 179-200
  9. Dimitar Dimitrov, Laurent Guillaud, Kohgaku Eguchi, Tomoyuki Takahashi
    Pages 201-215
  10. Matias Alloatti, Luciana Bruno, Tomas L. Falzone
    Pages 217-226
  11. Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Haham, Arnon Fluksman, Ofra Benny et al.
    Pages 239-250
  12. Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Haham, Cezary Marcinkiewicz, Peter I. Lelkes
    Pages 251-259
  13. Myrian Velasco, Carlos Larqué, Carlos Manlio Díaz-García, Carmen Sanchez-Soto, Marcia Hiriart
    Pages 261-273
  14. Micaela Pannella, Luciana Giardino, Laura Calzà, Mercedes Fernández
    Pages 275-294
  15. Francesco Gobbo, Fulvio Bonsignore, Rosy Amodeo, Antonino Cattaneo, Laura Marchetti
    Pages 295-314
  16. Hector Rosas-Hernandez, Elvis Cuevas, Susan M. Lantz, Merle G. Paule, Syed F. Ali
    Pages 315-331
  17. Maria Rosanna Bronzuoli, Roberta Facchinetti, Caterina Scuderi
    Pages 333-341
  18. Roberta Facchinetti, Maria Rosanna Bronzuoli, Caterina Scuderi
    Pages 343-352
  19. Gabriella Contarini, Pietro Giusti, Stephen D. Skaper
    Pages 353-360
  20. Vincenzo Sorrenti, Pietro Giusti, Morena Zusso
    Pages 361-372
  21. Serena Boccella, Francesca Guida, Enza Palazzo, Ida Marabese, Vito de Novellis, Sabatino Maione et al.
    Pages 373-378
  22. Irene Paterniti, Emanuela Esposito, Salvatore Cuzzocrea
    Pages 379-384
  23. Michela Campolo, Emanuela Esposito, Salvatore Cuzzocrea
    Pages 385-391
  24. Rosalia Crupi, Rosanna Di Paola, Emanuela Esposito, Salvatore Cuzzocrea
    Pages 393-401
  25. Rosa Espinosa-Luna, Alma R. Barajas-Espinosa, Fernando Ochoa-Cortez, Carlos Barajas-López
    Pages 423-432
  26. Asya Ozkizilcik, Roger Williams, Z. Ryan Tian, Dafin F. Muresanu, Aruna Sharma, Hari S. Sharma
    Pages 433-442
  27. Muge Yemisci, Secil Caban, Eduardo Fernandez-Megia, Yilmaz Capan, Patrick Couvreur, Turgay Dalkara
    Pages 443-454
  28. Back Matter
    Pages 455-458

About this book

Introduction

This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Neurotrophic Factors: Methods and Protocols, Second Edition is a valuable resource for a wide audience of readers as they explore nervous system function and pathology.

Keywords

Neurobiological research Animal models Cell vitality Neurons and glia Neurotrophin receptors Nervous system pathology

Editors and affiliations

  • Stephen D. Skaper
    • 1
  1. 1.Department of Pharmaceutical and Pharmacological SciencesUniversity of PadovaPadovaItaly

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7571-6
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7570-9
  • Online ISBN 978-1-4939-7571-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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